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《Journal of Xi'an Jiaotong University(Medical Sciences)》 2017-02
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Homologous SCCmec elements in clinical isolates of methicillin-resistant Staphylococcus epidermidis carrying psm-mec

YANG Yong-chang;XIAO Dai-wen;JIANG Wei;HUANG Wen-fang;Clinical Laboratory Department,Sichuan Academy of Medical Sciences;Sichuan Provincial People's Hospital;  
Objective To investigate SCCmec types in clinical isolates of methicillin-resistant Staphylococcus epidermidis(MRSE)carrying psm-mec.Methods We collected 165 strains of Staphylococcus epidermidis identified by automated microbiological identification system and screened MRSE by PCR amplification of esp and mecA gene.Strains with psm-mec were identified by amplification of psm-mec,fudoh and p221 DNA fragment;mec,ccr and SCCmec typing was conducted by multiplex PCR assay.Results Among 138 strains of MRSE,29 strains were identified as MRSE with psm-mec,and the carrying rate was 17.58%.Results of mec and ccr typing by multiple PCR showed that MRSE with psm-mec carried Class A mec,but the ccr type had obvious diversity.Results of SCCmec typing showed that all strains with psm-mec belonged to type Ⅱ and/or Ⅲ SCCmec.ConclusionClinical isolates of MRSE with psm-mec carry homologous typeⅡ and/orⅢ SCCmec harboring Class A mec.
【Fund】: 四川省卫计委课题资助(No.150218)~~
【CateGory Index】: R446.5;R440
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