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《Journal of Xi'an Jiaotong University(Medical Sciences)》 2017-02
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Neuroprotective effects of safranal on a rat model of traumatic injury to the spinal cord by anti-apoptotic,anti-inflammatory and edema-attenuating actions

ZHANG Chen;L Lei-feng;LI Miao;GAO Ling;MA Jun;WANG Kun-zheng;Department One of Orthopedics,the Second Affiliated Hospital of Xi'an Jiaotong University;Department of Ultrasound,the Second Affiliated Hospital of Xi'an Jiaotong University;  
Objective To investigate the effect of safranal on neurologic functions and histopathologic changes after spinal cord injury(SCI)and the related molecular mechanisms.Methods We randomly assigned 36 rats into six groups:control group,injury group and four treatment groups(namely,A,B,C,and D).The Basso Beattie Bresnahan locomotor rating scale(BBB)and HE staining were applied to evaluate the neuroprotective effect and determine the most effective dosage.Another 60 rats were randomly and evenly assigned to three groups:control group,injury group and treatment group.Nissl staining,TUNEL staining and electron microscopy were used to analyze histopathological changes;RT-PCR,immunohistopathological staining,ELISA,and Western blot were used to detect the expressions of apoptosis-related proteins(Bax and Bcl-2),inflammation-related factors(IL-1β,IL-10,TNF-αand P38MAPK),and edema-related factor(APQ-4).Results The optimal dosage for safranal was100 mg/kg.Neurocyte structure was found more distinct in treatment group than in injury group.In addition,we detected a smaller number of apoptotic neurocyte(26.37±1.54 vs.35.94±1.62,P=0.000),decreased Bax(P=0.000)and APQ-4[(359.55±16.12)% vs.(124.53±20.35)%,P=0.000]expressions,increased Bcl-2(P=0.036)expression,and obviously lowered P38 MAPK [(300.30±33.26)% vs.(132.54±10.21)%,P=0.000]expression.Conclusion Safranal exerts its neuroprotective function through anti-apoptosis,anti-inflammation and anti-edema in the rat model of spinal cord injury.
【Fund】: 国家自然科学基金资助项目(No.81301562);; 西安交通大学第二附属医院人才培养专项科研基金[No.RC(XM)201501]~~
【CateGory Index】: R285.5
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