Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Xi'an Jiaotong University(Medical Sciences)》 2017-02
Add to Favorite Get Latest Update

Sinomenine hydrochloride induces the apoptosis of hepatocellular carcinoma SMMC-7721 cells and its effect on expression levels of IL-1β,TNF and C/EBPβ

CAO Rui-qi;LU Xin-lan;REN Mu-dan;ZHANG Jun;LU Gui-fang;ZHANG Dan;ZHAO Yan;YAN Hong-lin;HE Shui-xiang;Department of Gastroenterolgy,the First Affiliated Hospital of Xi'an Medical College;Department of Gastroenterolgy,the First Affiliated Hospital of Xi'an Jiaotong University;  
Objective To observe the expression levels of IL-1β,TNF and C/EBPβduring the process of apoptosis induced by sinomenine Hydrochloride in hepatocellular carcinoma SMMC-7721 cells to clarify sinomenine hydrochloride'anti-tumor efficacy and its mechanism.Methods MTT assay was used to determine the in vitro effect of sinomenine hydrochloride on the growth of hepatocellular carcinoma SMMC7721 cells cultured.In in vitro cultured human hepatocellular SMMC7721 cells,quantitative analysis of apoptotic cells induced by sinomenine hydrochloride were assessed by flow cytometry after AnnexiV-FITC/PI staining.ELISA method was used to determine the expression levels of IL-1βand TNF-α,SMMC7721 cells were cultured for 48 hours with sinomenine hydrochloride 2 mmol/L,then the effects of sinomenine hydrochloride on C/EBPβwere detected by Western blot.Results Sinomenine hydrochloride inhibited the in vitro growth of human hepatocellular carcinoma SMMC7721 cells in concentration-and time-dependent manners as determined by MTT assay(P 0.05).After treatment with sinomenine hydrochloride of different concentrations(0,0.5,1and 2 mmol/L),the apoptosis rate of the cells was(8.31±0.81,12.62±1.13,27.43±0.97,and51.74±0.85)%,with significant difference between the experiment groups and control group(P0.05).The secretions of TNF-αand IL-1βwere decreased in supernatant of human hepatocellular carcinoma SMMC7721 cells.Sinomenine hydrochloride could significantly increase the C/EBPβprotein expression level(P 0.05).Conclusion Sinomenine hydrochloride inhibited the in vitro growth of human hepatocellar carcinoma SMMC7721 cells through apoptotic induction.This may be related to its decreasing the expression levels of IL-1β,TNF and C/EBPβand destroying tumor microenvironment during the process of apoptosis induced by dinomenine hydrochloride in hepatocellular varcinoma SMMC7721 cells.
【Fund】: 国家自然科学基金面上项目(No.81502095);; 陕西省教育厅专项科研计划项目(No.2013JK0786);; 陕西省社会发展科技攻关项目(No.2015SF55)~~
【CateGory Index】: R285
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 1 Hits
1 LIAO Hong-xia;WEI Yan-li;ZHU Xiao-yan;YE Jian;ZHOU Qi;L Hong-bin;Department of Ophthalmology,Institute of Field Surgery,Daping Hospital of the Third Military Medical University;Department of Ophthalmology,the Affiliated Hospital of Southwest Medical University;;The effect of FoxO1 inhibited by siRNA on the expression of IL-1β in diabetic rats retina induced by STZ and its mechanism[J];东南大学学报(医学版);2018-02
【Citations】
Chinese Journal Full-text Database 5 Hits
1 ;Advanced research on relationship between tumor microenvironment and radiosensitivity of tumor cells[J];吉林大学学报(医学版);2016-05
2 QIN Feng;CAI Hui;Department of Integrated Traditional Chinese and Western Medicine, Nanjing General Hospital of Nanjing Military Area Command;;Research Progress on Pharmacological Effects of Sinomenine[J];现代中药研究与实践;2016-04
3 ;Inhibition function of sinomenine against HepG2 cells proliferation[J];中医临床研究;2012-19
4 Xi Liang,Ya-Nan Hu,Shui-Xiang He,Ya-Xin Zhang,HeLei Hou,Zhan-Fang Zhu,Department of Gastroenterology,the First Affiliated Hospital of College of Medicine of Xi'an Jiaotong University,Xi'an 710061,Shaanxi Province,China Yuan Deng,Xiao-Feng Li,Department of Pathology,the First Affiliated Hospital of College of Medicine of Xi'an Jiaotong University,Xi'an 710061,Shaanxi Province,China;Significance of COX-2 and C/EBP β expression in hepatocellular carcinoma[J];世界华人消化杂志;2010-28
5 LIU Yan-yan,LIANG Shu-juan,WANG Huan-qin,ZHANG Su-hua,XIAO Wei-ling,WU Hui-na (Department of Immunology,Weifang Medical College; Key Immunology Laboratory of Higher Institution of Shandong Province,Weifang 261042,Shandong,China);Hepatocarcinoma specific IL-1β anti-sense RNA inhibits implanted hepatocarcinoma in mice[J];中国肿瘤生物治疗杂志;2009-03
【Co-citations】
Chinese Journal Full-text Database 8 Hits
1 WANG Yuan;LIU Chun-xiang;ZHANG Jun-hua;QIN Yi-shan;WANG Hui;LIU Yuan;Tianjin University of Traditional Chinese Medicine;Evidence-Based Medicine Center of Tianjin University of Traditional Chinese Medicine;;Treatment of ankylosing spondylitis with Zhengqing Fengtongning release tablets: Meta-analysis of randomized controlled trials[J];中国中药杂志;2018-16
2 ZENG Xi-yao;YANG Hua;College of Biological Science and Technology,Hunan Agricultural University;Plant Science National Level Experimental Teaching Demonstration Center,Hunan Agricultural University;;Advances of Chemical Constituents,Pharmacological Activities and Cultivation and Breeding in Sinomenum acutum[J];湖南生态科学学报;2018-01
3 ZHANG Qing-wei;Pingyi County Hospital of Traditional Chinese Medicine of Shandong Province;;Analysis of the Application of Rattan Chinese Herbal Medicine in the Outpatient Department of Our Hospital[J];世界最新医学信息文摘;2018-22
4 GENG Rong-hui;WANG Yu-qing;Nanyang Medical College;;Quality standard for compound Tripterygium hypoglaucum gel[J];中成药;2018-02
5 LIU Liang;HU Yue-yang;JU Ying-chao;ZHOU Rong-miao;Tumor Institute,Forth Hospital of Hebei Medical University;Laboratory Animal Center,Forth Hospital of Hebei Medical University;;Expression of long noncoding RNA(linc-VLDLR) in extracellular vesicles and its relation to the development and multidrug resistance of esophageal cancer[J];解放军医学杂志;2018-01
6 Li Li;Hu Xiyao;Wan Chao;Guo Lihong;Wu Shengying;Hubei University of Medical Hubei,Lab of Medical functions;Department of acupuncture and moxibustion, Taihe Hospital, Hubei Medical College;;Effect of Sinomenine on the Healing of Spinal Compression Fracture in Rabbits[J];世界科学技术-中医药现代化;2017-12
7 JIANG Qie-ying;TANG Tao;LIAO Zheng-gen;DONG Wei;ZHANG Jing;ZHAO Guo-wei;KONG Xiao-qiang;LIANG Xin-li;Laboratory Animal Science and Technology Center,Jiangxi University of Traditional Chinese Medicine;Key Laboratory of Modern Preparation of Traditional Chineses Medicine,Ministry of Education,Jiangxi University of Traditional Chineses Medicine;;Study on the Correlation between Effective Component of Traditional Chinese Medicine and the Function of Reversing Multidrug Resistance of Tumor Cells[J];江西中医药大学学报;2017-06
8 YU Yang;ZHANG Cui-ping;WANG Shu-xia;The Affiliated Hospital of Qingdao University;;COX-2 Protein Expression and Its Relation to Clinicopathological Features of Hepatocellular Carcinoma:A Meta Analysis[J];中国肿瘤;2017-11
【Co-references】
Chinese Journal Full-text Database 7 Hits
1 CAO Rui-qi;LU Xin-lan;REN Mu-dan;ZHANG Jun;LU Gui-fang;ZHANG Dan;ZHAO Yan;YAN Hong-lin;HE Shui-xiang;Department of Gastroenterolgy,the First Affiliated Hospital of Xi'an Medical College;Department of Gastroenterolgy,the First Affiliated Hospital of Xi'an Jiaotong University;;Sinomenine hydrochloride induces the apoptosis of hepatocellular carcinoma SMMC-7721 cells and its effect on expression levels of IL-1β,TNF and C/EBPβ[J];西安交通大学学报(医学版);2017-02
2 HAN Gai-ling;Department of Endocrinology,the First Hospital of Qinhuangdao City;;Correlation of connective tissue growth factor and 25-hydroxy vitamin D levels with retinopathy in type 2 diabetes[J];现代医学;2016-08
3 Zhou Yingni;Wang Qingzhu;Qin Guijun;Guo Feng;Zhang Yuanyuan;Wu Lina;Department of Endocrinology and Metabolism,the First Affiliated Hospital of Zhengzhou University;;Effect and mechanism of FoxO1 overexpression in rats with diabetic nephropathy[J];中华内分泌代谢杂志;2015-02
4 Li Nana;Ren Shouan;Shanxi Medical University;;Effects of chronic intermittent hypoxia on IRS-2 and FoxO1 expression in rat heptocytes[J];国际内分泌代谢杂志;2015-01
5 Liu Xiaojing;Zhu Bijun;Zou Haidong;Hu Daode;Qiu Yating;Gu Qing;Liu Kun;Xu Xun;Department of Ophthalmology,Shanghai First People's Hospital,Affiliated Shanghai Jiaotong Univesity;;Carbamylated erythropoietin-mediated protective effect on diabetic retinopathy and underlying mechanism[J];中华实验眼科杂志;2014-11
6 DIAO Xue-hong,SHEN E,ZHANG Yue-li,HU Bing,ZHANG Jing-an,WU Zuo hui,WEI Cong (Department of Ultrasound in Medicine,Affiliated Sixth People's Hospital of Shanghai Jiaotong University,Cardiovascular Disease Laboratory,Shanghai Institute of Ultrasound in Medicine,Shanghai 200233,China.);Inhibition of Rac1 protects cardiac functions in STZ-induced diabetic cardiomyopathy through decreasing pho-p38 MAPK[J];中国病理生理杂志;2010-07
7 LI Yong-hao,LU Lin,CHEN Lin-yan,et al.State Key Laboratory of Ophthalmology,Zhongshan Ophthalmic Center,Sun Yat-sen University,Guangzhou 510060,China;Protective effect of blocking the signal path of p38 mitogen-activated protein kinase on blood-retinal barrier and retinal ganglion cells in early diabetic rats[J];中华眼底病杂志;2010-02
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Jing Wang;Zi-Rong Yang;Wei-Guo Dong;Ji-Xiang Zhang;Xu-Feng Guo;Jia Song;Shi Qiu;Department of Gastroenterology, Renmin Hospital of Wuhan University;;Cooperative inhibitory effect of sinomenine combined with 5-fluorouracil on esophageal carcinoma[J];World Journal of Gastroenterology;2013-45
2 Yang Guocai,Yu Shan,Xu Guangquan.;Sinomenine inhibits proliferation of A549 human lung cancer cells[J];中国中西医结合外科杂志;2011-05
3 TIAN Yun-hong,XIE Guo-zhu,REN Chen,SUN Quan-quan,SUN Ai-min,LIU Ying,YUAN Ya-wei Department of Radiation Oncology,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China;Radiation-induced G2 phase arrest may contribute to the radioresistance of breast cancer stem cells[J];南方医科大学学报;2011-01
4 QI Ya-li,WANG Jun,LI Yan,WANG Hong-yan,GONG Shou-liang. Key Laboratory of Radiobiology,Ministry of Health,School of Public Health,Jilin University,Changchun 130021,China;Effect of ionizing radiation combined with inhibitors or inducer of autophagy and apoptosis on MCF7 cells[J];中华放射医学与防护杂志;2010-03
5 Jun-Xian Wang,Kai-Guang Zhang,Department of Gastroenterology,Anhui Provincial Hospital Affiliated to Anhui Medical University,Hefei 230001,Anhui Province,China;Advance in relationship between COX-2 and gastric cancer[J];世界华人消化杂志;2009-04
6 SUN Jian-xiang, SUN Wei-jian, SUI Jian-li, et al.Beijing Institute of Radiation Medicine, Beijing 100850, China;Correlation between residual level of DNA double-strand breaks and the radiosensitivity of cancer cells[J];中华放射医学与防护杂志;2008-05
7 Bao-Feng Wang, Yan-An Cheng, Xi-Jing Wang, Zhen-Guo Liu, Hong-Bing Ma, Ming-Hua Bai, Shuang-Suo Dang, Xiao-Li Jia Department of Oncology, the Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710004, Shaanxi Province, China Department of Infectious Diseases, the Second Hos-pital of Xi’an Jiaotong University, Xi’an 710004, Shaanxi Province, China;Inhibitory effect of saikosaponin-d on the angiogenesis of experimental hepatocarcinoma in rats[J];世界华人消化杂志;2008-12
8 XIAO Wei-ling,LIANG Shu-juan,MU Dong-zhen,WANG Xue-jing,WU Hui-na Key Laboratory of Molecular Immunology,Weifang Medical College,Weifang 261042,China;Expression of recombinant mouse IL-1β significantly down-regulates NK cell mediated cytotolysis against H22 hepatoma cells[J];细胞与分子免疫学杂志;2007-12
9 Nattiya Hirankarn Immunology Unit,Department of Microbiology,Faculty of Medicine,Chulalongkorn University,Bangkok 10330,Thailand Ingorn Kimkong Pittaya Kummee Inter-Department of Medical Microbiology,Graduate School,Chulalongkorn University,Bangkok 10330,Thailand Pisit Tangkijvanich Department of Biochemistry,Faculty of Medicine,Chulalongkorn University,Bangkok 10330,Thailand Yong Poovorawan Department of Pediatrics,Faculty of Medicine,Chulalongkorn University,Bangkok 10330,Thailand;Interleukin-1β gene polymorphism associated with hepatocellular carcinoma in hepatitis B virus infection[J];World Journal of Gastroenterology;2006-05
10 De-Kai Qiu Xiong Ma Yan-Shen Peng Xiao-Yu Chen Shanghai Institute of Digestive Diseases,Renji Hospital,Shanghai Second Medical University,Shanghai,200001,China;Significance of cyclooxygenase-2 expression in human primary hepatocellular carcinoma[J];World Journal of Gastroenterology;2002-05
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved