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《Journal of Xi'an Jiaotong University(Medical Sciences)》 2017-02
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Effects ofAralia on apoptosis and expression of apoptosis-related genes in hepatic stellate cells

SHI Hai-tao;LIU Xin;LIU Chao;HUANG Miao;JIANG Jiong;CHENG Yan;ZHAO Gang;DONG Lei;Department of Gastroenterology,the Second Affiliated Hospital of Xi'an Jiaotong University;Department of Gastroenterology,the First Hospital of Yulin;Department of Nephrology,the First Affiliated Hospital of Xi'an Jiaotong University;  
Objective To investigate the effects of Aralia on apoptosis and expression of apoptosis-related genes in hepatic stellate cells(HSC-T6)and to explore the anti-fibrotic mechanism of Aralia.Methods We divided 30 male SD rats at random into negative control group,colchicine-treated and aralia-treated groups,with10 in each.After administered with normal saline,colchicine and aralia by gavage separately for 3 consecutive days,serum containing the drug was prepared.Then HSC-T6 cells were treated with serum containing the drug mentioned above for 48 h.Flow cytometry was used to assess apoptosis.Reverse transcription PCR(RT-PCR)was used to detect mRNA expressions of Bcl-2,Bcl-xs,Bax and Bcl-xl.And the protein expressions of Bcl-2,Bax andα-SMA were determined by Western blot.Results 1 The apoptosis rates of HSCs in colchicine-treated and Aralia-treated groups were significantly higher than that in negative control group(P0.05);Aralia-treated group had a higher apoptosis rate than colchicine-treated group(P0.05).2 Colchicine and Aralia could decrease Bcl-2 expression,but increase Bax expression markedly(P0.05);and Aralia exerted a more obvious effect.3 There was no significant difference in the mRNA expressions of Bcl-xs and Bcl-xl among the three groups.4 Colchicine and Aralia could inhibitα-SMA expression evidently(P0.05),and the effect was more obvious in Aralia-treated group.ConclusionAralia can significantly accelerate the apoptosis of HSC-T6 cells possibly by up-regulating Bax expression and downregulating Bcl-2 expression.
【Fund】: 陕西省社会发展攻关计划资助项目(No.S2011SF1610)~~
【CateGory Index】: R285.5
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