Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Cellular and Molecular Immunology》 1986-04
Add to Favorite Get Latest Update

Detections of HFRS virus antibodies in various sera from epidemic area of the house rat type of HFRS by a McAb-ELISA indirect sandwich method

Xu Zhi-kai et al (Department of Microbiology, Fourth Military Medical College, Xian)  
HFRS virus IgM and/or IgG antibodies in human and several animals sera drawn from Shanxi Province, which was the epidemic area of the house-rat type of HFRS, were detected by a McAb-ELISA indirect sand wich method and IFAT or enzyme-labeled SpA staining test. The IgM antibody positive rates of 140 HFRS patients' sera detected by the ELISA and IFAT were 65.71% and 45%, respectively, and the difference was significant(P0.01). The IgG antibody positive rates of 174 HFRS patients' sera detected by the ELISA and IFAT were 80.46% and 67.24%, respectively, and the former was higher than the latter(P0.01). The titer measured by ELISA, either of IgM or IgG, was higher than the titer by IFAT(P0.01). The positive rate of 295 sera from health individuals who had not history of HFRS detected by the ELISA was higher than one by IFAT(P0.01) . The positive rates of 215 sera of the rat, 102 sera of the rabbit and 108 sera of the pig detected by the ELISA were similar to those by IFAT or enzyme-lebeled SpA staining test(P0.05). The specificity of the antibody detected by the ELISA was proved by the antibody-blocking test. Several parameters of the ELISA were disscused. The analytical result shows that the McAb-ELISA is a specific, sensitive and simple method, and can be used for early diagnosis, definit and retrospect diagnosis and epid-emiological investigation of HFRS.
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 1 Hits
1 Xu Zhikai Wang Meixian Jiang Shaochun Ma Wenyu (Department of Microbiology, The Fourth Military Medical College, Xian);DETECTION OF EPIDEMIC HEMORRHAGIC FEVER VIRUS ANTIGEN BY A DOUBLE MONOCLONAL ANTIBODY ELISA INDIRECT SANDWICH METHOD[J];Chinese Journal of Virology;1986-04
Chinese Journal Full-text Database 10 Hits
1 Huang Qiquan Wang Meixian (Department of Microbiology, The Fourth Military Medical College, Xian);PROTECTION OF MONOCLONAL ANTIBODY FOR HFRS VIRUS INFECTED MICE[J];Chinese Journal of Virology;1990-02
2 Wang Liya, et al. Department of Microbiology;Establishment and Identification of Hybridoma Cell Lines Secreting Monoclonal Antibodies against Hemagglutinin of Hemorrhagic Fevev with Renal Syndrome Virus[J];Journal of The Fourth Military Medical University;1988-01
3 Xu Zhikai, et al. Department of Microbiology;Detection of HFRS Virus Antibodies in Human and Animal Sera from Epidemic Area of the House-Rat Type of HFRS by a McAb-ELISA Indirect Sandwich Method[J];Journal of The Fourth Military Medical University;1988-03
4 Wang Meixian, et al. Department of Microbiology;The Study and Application of the Monoclonal Antibodies against Haemorrhagic Fever with Renal Syndrome Virus[J];Journal of The Fourth Military Medical University;1988-04
5 Xu Zhikai, et al. Department of Microbiology;Detection of the Antigens in Athymic Nude Mice Infected by Hemorrhagic Fever with Renal Syndrome Virus[J];Journal of The Fourth Military Medical University;1988-04
6 Xu Zhikai, Wang Haitao, Wei Lixin and Wang Meixian Department of Microbiology;Preliminary Study on the Immunological Characteristics of HFRS Virus 50K Structural Protein[J];Journal of The Fourth Military Medical University;1989-04
7 Bai Xuefan el al Department of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an;Comparison of Three ELISA Methods for Detecting Hemorrhagic Fever with Renal Syndrome Virus Antigens[J];Immunological Journal;1990-01
8 Liu Qiju et at Department of Microbiology,Third Military Medical University, Chongqing;Application of Microplaque Immunoperoxidase Detection of Hemorrhagic Fever with Renal Syndrome Virus and Specific Antibody[J];Immunological Journal;1990-03
9 Zhang Yiju et al;The Preparation of Epidemic Hemorrhagic Fever Virus Antigen of ELISA[J];Chinese Journal of Zoonoses;1989-01
10 Xu Haifeng et al (The Fourth Military Medical University, Tangdu Hospital, Xian, 710000);Protection Against HFRS Virus Infection in Suckling Mice by Transfering Monoclonal Antibodies[J];Chinese Journal of Zoonoses;1993-06
Chinese Journal Full-text Database 2 Hits
1 Zhang Yun;Haemolaelaps Glasgowi Discovered to be a Mite-borne Natural Vector of EHF Virus[J];Jiangsu Medical Journal;1985-06
2 Yang Zhanqing,et al.,Army Military Medical Research Institute, Jinan;Study on Typing of Endemic Areas of Epidemic Hemorrhagic Fever with A-and R-hemagglutinin Antigens[J];;1990-04
【Secondary References】
Chinese Journal Full-text Database 3 Hits
1 YANG Zhan-qing1, YU Shou-yi1, NIE Jun1, CHEN Qing1, LI Zhi-feng1, LIU Yun-xi2, ZHANG Jin-lan2, XU Jian-jiang2, YU Xiao-ming2, BU Xiu-ping2, SU Jing-jing2, ZHANG Yun3, TAO Kai-hua31Department of Epidemiology, Southern Medical University, Guangzhou 510515, China; 2Institute of Military Medicine of Jinan Command, Jinan 250014, China; 3Institute of Military Medicine of Nanjing Command, Nanjing 210002, China;Prevalence of hemorrhagic fever with renal syndrome virus in domestic pigs: an epidemiological investigation in Shandong province[J];Journal of First Military Medical University;2004-11
2 YANG Guo-ping1,LIU Yan-qiu2,XIE Huai-jiang1,WANG Zhong-hui3,REN Qing-ming1,LIU Guo-ping1,WANG Feng1,ZHAO Yue3,WANG Yin-ping1,YU Ning1,ZHOU Qing-yang1,YANG Qing1,LIU Jiang-qiu1(1.Disease Prev.& Cont.Cent.,of Shenyang Milt.Command,Shenyang 110034;2.Disease Prev.& Cont.Cent.,of Ji'nan Milt Command Ji'nan 250000;3.Zhejiang Weixin Biology,Ningbo 315600);Biological Characters of Six HFRS Virus Strains from Three Provinces in Northeast China[J];Journal of Microbiology;2008-05
3 YANG Zhan-qing, YU Shou-yi, NIE Dong, CHEN Qing, LI Jian-jun, LI Zhi-feng, LIU Yun-xi, ZHANG Jing-lan, YU Xiao-min, SU Jing-jing, LI Jing, CHEN Shao-ling, ZHANG Yun, TAO Kai-hua, ZHANG Li-ping Department of Epidemiology, South Medical University, Guangzhou, 510515 Center for Disease Prevention and Control of Jinan Military Command, Jinan, 250014 Center for Disease Control and Prevention of Nanjing Military Command, Nanjing, 210000; Department of Pharmacy, General Hospital of Jinan Military Command, Jinan, 250014.;Molecular epidemiological investigation of relationship between HV natural infection and HFRS in pigs[J];Medical Journal of National Defending Forces in Southwest China;2006-03
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 An Xianlu, Xu Zhikai, Li Enshan and Zhen Rongfen.Director: Wang Meixian. Fourth Military Medical College, Xi-anJiang Kejian and Gan Rihuai. Health and Anti-epidemic Station of Shanxi, Xi-an;ESTABLISHMENT AND IDENTIFICATION OF HYBRIDOMA CELL OF MONOCLONAL AN-TIBODIES FOR VIRUS OF HEMORRHAGIC FEVERS WITH RENAL SYNDROME(HFRS)[J];Medical Journal of Chinese People's Liberation Army;1984-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved