Experimental Study on Identification of Salmonella Typhimurium Using Monoclonal Antibodies
Li Biehu LI Yuan Yang Xiaofeng Li Dongyang et al(Department of Microbiology, 4th Military Medical University, Xi'an 710032)
We developed an ELISA method using monoclonal antibodies against Salmonella typhimurium ( McAb, STM) for detection of STM. 162 clinical isolates of STM were investigated by ELISA and staphylococcus protein A coagglutination test ( SPA- CoA) simultaneously, and a comparison was made between these two methods. The results indicated that the ELISA method was sensitive enough to detect 5×102/ml of STM, and its specificity was also high; the detection rate of STM 0-4 antigen is 100% while that of H-i antigen is 96.3%. Furthermore, this study revealed that the sensitivity and specificity of the McAbs used were all comparable to the current standard of the diagnostic serum factor for Salmonella. The experimental method showed a promising prospect of application.