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《Journal of Cellular and Molecular Immunology》 1992-01
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The Comparison of IL-2 and IL-6 Activity Level in a Variety of Feeder Cell Culture Supernatants and Conditioned Media for Hybridoma Technique

Yang Kun Jin Boquan Zhao Ning Zhu Yong Liu Ying Liu Xuesong (Department of Immunology, 4th Military Medical University, Xian 710032)  
The IL-2 and IL-6 biological activities in feeder cell culture supernatants,or conditioned media for hybridoma technique were detected by cytokine dependent cell 3H-TdR incorporation methods. The results indicated that these samples contained no detectable IL-2 except supernatants from Rat mixed thymocyte culture and the levels of IL-6 activity were 10 000u/ml, 2 512u/ml, 158u/ml, 126u/ml, 79u/ml and 16u/ml in supernatants from CRL1506, endotheliocyte, Rat mixed thymocyte, macrophage, splen-ocyte and thymocyte culture, respectively. The main role of feeder cells and conditioned media for hybridoma technique was discussed.
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