Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Cellular and Molecular Immunology》 1992-01
Add to Favorite Get Latest Update

The Comparison of IL-2 and IL-6 Activity Level in a Variety of Feeder Cell Culture Supernatants and Conditioned Media for Hybridoma Technique

Yang Kun Jin Boquan Zhao Ning Zhu Yong Liu Ying Liu Xuesong (Department of Immunology, 4th Military Medical University, Xian 710032)  
The IL-2 and IL-6 biological activities in feeder cell culture supernatants,or conditioned media for hybridoma technique were detected by cytokine dependent cell 3H-TdR incorporation methods. The results indicated that these samples contained no detectable IL-2 except supernatants from Rat mixed thymocyte culture and the levels of IL-6 activity were 10 000u/ml, 2 512u/ml, 158u/ml, 126u/ml, 79u/ml and 16u/ml in supernatants from CRL1506, endotheliocyte, Rat mixed thymocyte, macrophage, splen-ocyte and thymocyte culture, respectively. The main role of feeder cells and conditioned media for hybridoma technique was discussed.
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 1 Hits
1 LI Li~△,YANG Yi,ZHANG Yu,et al (()~△Changchun Institute of Biological Products,Changchun 130062,China);Rescue of Dying Adherent Cells by Feeder Cells[J];Chinese Journal of Biologicals;2007-02
Chinese Journal Full-text Database 7 Hits
1 Ai Xi;Ye Jianxin; Xiao Chengzu;Zhang Zhengguang(Institute of Biotechnology, Academy of Military Medical Sciences , Beijing 100071);Preparation and characterization of monoclonal antibodies against uPA and their application for purification of uPA[J];BULLETIN OF THE ACADEMY OF MILITARY MEDICAL SCIENCES;1995-04
2 Huang Xiaohua;Chen Jing(Wuhan Institute of Biological Products,Whuan 430060);Establishment of a Sandwich ELISA with Double Antibodies to Human Serum Fibronectin[J];JOURNAL OF CELLULAR AND MOLECULAR IMMUNOLOGY;1995-Z1
3 Liu Mingxu, Wang Fusheng, Jin Lei, Hong Weiguo, et al. Institute of Infectious Diseases of 302 Hospital, Beijing 100039, P. R. China;Genotyping and Polymorphism of CCR5A32, CCR2b - 641 and SDFl - 3' A Alleles in Uygur Population in Xinjiang[J];Journal for China AIDS/STD;2000-06
4 Chen Renbiao Ye Genyao Geng Zhencheng Wang Zenghui Kong FanhuaTian Ding Bao Piyun Liu Ruoying Liu Jie Song FangjiFan Lian Zhang Gongliang Guo ShishiXu Linmin Xu Xingpei Cheng DingzhenZhao Xiuzhu( Shanghai Institute of Immunology, Shanghai Second Medical University, WHO Collaborating Center lor Immunogenetics and Immunopathology; Beijing North Taiping Road Hospital; Genetics Institute, Fudan University; Neuroscience Institute, Guangzhou Medical College; Xuanwu Hospital, Capital Medical Col-left; Inner Mongolia Medical College; Guiyang Medical College; Haer-bin Red Cross Blood Center; No. 1 Affiliated Hospital, China Medical University; Shanghai Institute of Blood Transfusion; Hunan Medical University; Beijing Institute of Paediatrics; Wuhan Institute of Biological Produts; No. 1 Affiliated Hospital, Xinjiang Medical College; Sin-o-Australian Friendship Complement Laboratory, Tongji Medical university);Revelations of the Origin of Chinese Nation from Clustering Analysis and Frequency Distribution of HLA Polymorphism in Major Minority Nationalities in Mainland China[J];Acta Genetica Sinica;1993-05
5 HONG Wei-guo, WANG Zhe, WANG Fu-sheng, et al Institution of Infectious Disease of 302 Hospital ,Beijing 100039, China;Polymorphisms and Implications of HIV-1 Relevant Alleles in Patients with Sexually-Transmitted Diseases and HIV-1 Carriers[J];The Chinese Journal of Dermatovenereology;2001-04
6 WANG Fusheng 1, LIU Depei 2. 1(Department of Bioengineering, Beijing Institute of Infectious Diseases, Beijing, 100039 P. R. China. E mail: fushengw@public.bta.net.cn); 2(National Key Laboratory of Molecular Biology, Institute of Basic Medicine,;Polymorphism of human alleles associated with genetic resistance against HIV-1 infection and its implications[J];CHINESE JOURNAL OF MEDICAL GENETICS;2000-04
7 Dong Jiaxin,Sun Yingxun,Yu Ming et al.Institute of Basic Medical Science,Academy of Military Medical Sciences,Biejing 100850;PRODUCTION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST RECOMBINANT HUMAN INTERLEUKIN 6 RECEPTOR[J];CHINESE JOURNAL OF IMMUNOLOGY;1997-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved