Construction and identification of eukaryotic expression vectors bearing the gene of human antibody against HFRS virus
Wu Xing an, XU Zhi kai, JIANG Shao zhun, YAN Yan, LUO Wen, BAI Wen tao, ZHANG Fang lin, LIU Yong, WANG Hai taoDepartment of Microbiology,Fourth Military Medical University,Xi'an 710032, Shaanxi Province, China
Aim To construct anti HFRS virus human engineering antibody expression vectors and then to express it in eukaryotic cells. Methods The variable region genes of human anti HFRS virus antibody were connected with human antibody constant region genes,antibiotic resistant gene and elements of eukaryotic expression regulation including promotor,leader sequence,enhancer and splice signal via several times cloning. Thus human antibody eukaryotic expression vectors VHEpSV gpt and VκEpSV neo were constructed respectively. Results Variable region genes of human anti HFRS virus antibody were connected with the vectors pSV gpt and pSV neo by enzyme digestion. Conclusion Eukaryotic expression vectors of human anti HFRS virus antibody are constructed successfully by using engineering technoigue.