Detection of HPV infection in larynx squamous cell carcinoma tissues by indirect in situ polymerase chain reaction
LI Xiao,WU Neng ding,XU Ru jung, SHEN Bao zhu, LI Xiang hongDepartment of Pathology, First Municipal Hospital of Hangzhou, Hangzhou 310006, Zhejiang Province, China
Aim To make up a stable method of in situ PCR and to probe the relationship between HPV infection and larynx squamous cell carcinomas. Metheds HPV infection in 50 specimens of larynx squamous cell carcinoma was examed by immunohistochemical staining. in situ hybridization,PCR and indirect in situ PCR. Results The positive detection rate of HPV DNA was 12%by immunohistochemistry, 26%by in situ hybridization,20%by PCR, 34%by in situ PCR, and totally 42%in twenty one out of fifty cases. Conclusion HPV infection is related to the occurrence of larynx squamous cell carcinomas. Indirect in situ PCR has better sensitivity,reliability, and specificity than other methods. It is reliable and has the role of diagnosis of localization and nature of the lesion.