Influence of bifidobacterium DNA on PKC and NF-κB in murine macrophages
WANG Li-sheng~1, LI Ying-xue~1, ZHU Hui-ming~1, ZHU Zhong-sheng~1, MA Xiao-dong~2 ~1Department of Gastroenterology, the Second Clinical Medical Hospital, Jinan University, Shenzhen 518020; ~2Center Laboratory, Nanfang Medical University, Guangzhou 510515, China
AIM: To explore the influence of DNA of bifidobacteriua adolescence on PKC and NK-κB of murine macrophages. METHODS: The fluorescent intensity of PKCα、 PKCβI、 PKCβII、 PKCγ、 PKCε and PKCζ in murine peritoneal macrophages was detected by using laser confocal microscope. The density of NK-κB~+ macrophages was detected by immunocytochemical staining. RESULTS: The average fluorescent intensity of PKCα and PKCβII produced by mouse peritoneal macrophages in bifidobacterium DNA injection group was markedly higher than that in control group(P0.01). There was no statistically significant difference of average fluorescent intensity of PKCβI、 PKCγ、 PKCε and PKCζ between the two groups(P0.05). The density of NK-κB~+ macrophages in bifidobacterium DNA injection group was markedly higher than that in control group(P0.01). CONCLUSION: DNA of bifidobacteria adolescence could activate macrophages by promoting the activity of PKCα、 PKCβII and NF-κB.