Induction and mechanism study of anti-melanoma immune response by M.tuberculosis Ag85B
WU Xue-zhong, WEI Hai-ming , ZHENG Xiao-dong, JIN Teng-chuan, TIAN Zhi-gang Shandong Provincial Institute of Dermatology and Venereology, Jinan 250022,China
AIM: To study the anti-melanoma immunity efficacy of Ag85B antigen gene therapy in vivo. METHODS: C57BL/6 mice were inoculated s.c. with B16 cells, and 8 days later the mice were inoculated s.c. again with B16 cells (control group 1), B16/pcDNA3 cells (control group 2) or B16/pcDNA3-Ag85B cells (experimental group), respectively. Tumor volume, survival time, serum IFN-γ level and IL-4 level of 3 groups mice were observed. Antitumor activity of Ag85B was studied. RESULTS: From 12 to 23 day, the mean tumor volume of mice increased from 1.1058 cm~3 and 0.9123 cm~3 to 7.5983 cm~3 and 5.8746 cm~3 in the control group 1 and 2, respectively. But it increased from 0.5158 cm~3 to 1.5080 cm~3 in the experimental group. The mean survival time of mice was 24.1 days and 24.7 days in the control group 1 and 2, respectively. That was 27.8 days in the experimental group. Within 13 days after the last inoculation, the serum IFN-γ level of all groups experienced increased (That increased to26.3ng/L,23.0ng/L and 25.2ng/L in the control group1,2 and the experimental group, respectively). Subsequently, the serum IFN-γ level in the two control groups decreased (That decreased to 19.3 ng/L and 18.3 ng/L in the control group 1 and 2) while it still augmented in the experimental group(That increased to 46.5 ng/L). IL-4 level was slightly but not significantly enhanced and then declined in all mice.CONCLUSION: Ag85B induced the increase of serum IFN-γ level in the animals experiments , inhibited the tumor growth and prolonged the survival of the tumor-bearing mice.