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《Chinese Journal of Cellular and Molecular Immunology》 2007-01
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EffectofST6GalIsiRNA-mediatedgene silencing on the adhesion and invasion of SW480 cells

YUAN Tian-hong, LI Ming-yuan , LI Wan-yi, LI Hong, JIANG Zhong-hua Department of Microbiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China  
AIM: To study the effect of synthesized ST6Gal I specific siRNA on the adhesion and invasiveness of human colon carcinoma cell line SW480 with over expression of ST6Gal I. METHODS: A double strand small interference RNA (siRNA) targeting ST6Gal I was designed and synthesized, and then transfected into SW480 cells by lipofectmine 2000. SW480 cells were cultured and divided into 4 groups: blank control group, liposome control group, non-specific siRNA group and ST6Gal I siRNA group. The expression of ST6Gal I mRNA was examined by RT-PCR and the amount of α-2,6-sialylation on the SW480 cell surface was detected by flow cytometry. The adhesion and invasion of SW480 cells to extracellular matrix (ECM) were analyzed by using CytoMatrix~ TM kit and cell invasion assay kit, respectively. RESULTS: After SW480 cells were transfected for 48 hours, the expression of ST6Gal I mRNA in ST6Gal I siRNA group was significantly decreased compared with that in the blank control group, liposome control group, and non-specific siRNA group (P0.05). After SW480 cells were transfected for 72 hours, the amount of α-2,6-sialylation on cell surface, the adhesion and invasion of the cells in ST6Gal I siRNA group were markedly lower than those in the other 3 groups (P0.05). CONCLUSION: The chemically synthesized specific siRNA targeting ST6Gal I can effectively inhibit the expression of ST6Gal I and reduce cell adhesion and invasion to ECM in SW480 cells. Our research is important for further study of anti-tumor treatment with RNA interference.
【Fund】: 国家教育部博士点基金资助项目(20040610050);; 贵阳市科技局基金资助项目(T2004-8)
【CateGory Index】: R735.3
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