Effect of daqingye on EP3 mRNA expression of hypothalamus in IL-1β-induced rabbits
DONGJun~ 1,2* ,QIU Sheng~1, XIE Xin-hua~1,PANRui~3, TANG Hong-mei~1, LU Da-xiang~ 1, 2 ~1Department of Pathophysiology, ~2Key Lab of State Administration of Traditional Chinese Medicine, Medical College of Jinan University, Guangzhou 510632; ~3Department of Orthopaedics, the First Affiliated Hospital of Jinan University, Guangzhou 510630, China
AIM: To study the antipyretic effect and mechanism of Daqingye injection (DQYI). METHODS: The IL-1β-induced febrile New Zealand rabbits were chosen as experimental models and the antipyretic effect of DQYI was observed. The expression of EP3 mRNA was investigated by using in situ hybridization (ISH) in POAH. RESULTS: First, the colonic temperature went up gradually after intravenous(i.v) IL-1β. The peak value of temperature(△T) and thermal response index (TRI_1) in IL-1β-treated group were higher than those in control group (P0.01). The △T and TRI_1 of in DQYI and IL-1β-treated group were lower than those in IL-1β-treated group (P0.05). The temperature of DQYI-treated group showed no distinguished difference compared with that in control group (P0.05). Second, the expression of EP3 mRNA in the POAH of IL-1β-treated group increased markedly compared with that in control group (P0.01). The expression of EP3 mRNA treated by IL-1β+DQYI in the POAH decreased strikingly compared with that in IL-1β group(P0.05). CONCLUSION: DQYI has distinct antipyretic effect on IL-1β-induced fever. The mechanism might be the inhibition of EP3 mRNA expression in POAH from rabbits.