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Effects of Dengue virus infection on PGIS expression and PGI_2 secretion of human vascular endothelial cells

JIANG Zhen-you~1, XIAO Rui~ 1, 2 Department of Microbiology and Immunology, Medical College of Jinan University, Guangzhou 510632, China  
AIM: To elucidate the effects of Dengue virus on the expression and secretion of prostacyclin (PGI_2) in endothelial cells. METHODS: Cultured human umbilical vein endothelial cells (HUVEC) were infected by Dengue virus I I(DV_2)for different duration. PGI_2 level in the supernatant was determined by radioimmune assay. Cytoplasmic RNA of the infected HUVEC was prepared using the Trizol method and was assayed for prostacyclin synthase (PGIS)by RT-PCR. RESULTS: PGIS expression as well as PGI_2 secretion of the DV_2 group were significantly increased (P0.05) at 48 h, 72 h and 96 h post-infection compared to the control group. CONCLUSION: DV_2 could promote the expression of PGIS mRNA in HUVEC and increase the level of PGI_2, which may increase the vascular permeability. The dysfunction of vascular endothelial cell (EC) induced by DV may be related to the pathogenesis of Dengue haemorrhagic fever (DHF)and Dengue shock syndrome(DSS). [
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