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Induction of specific CTL by DC from axillary lymph node of patients with breast cancer

LIU Yun-jiang~1, ZHANG Jian-li~1, SHAN Bao-en~ 2* ~1Department of Surgery, ~2Research center, the Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China  
AIM: To investigate the capability of the dendritic cells (DC) from axillary draining lymph node of patients with breast cancer to induce the antigen specific CTL. METHODS: The mononuclear cells were isolated from axillary draining lymph node, and the adherent cells were cultured with rhGM-CSF and rhIL-4 to induce DC. The non-adherent cells were cultured with IL-2 to induce tumor draining lymph node cells(TDLNC). DCs stimulated by the autoallergic breast cancer freeze-thawing antigen were co-cultured with the obtained TDLNC to induce tumor antigen specific CTL. RESULTS: The positive percentage of CD1a, CD83, CD86 on DC from freshly isolated mononuclear cells in axillary draining lymph node was 11.0±2.3, 26.6±5.2 and 33.0±6.1, respectively. After co-cultured with rhGM-CSF, rhIL-4, breast cancer freeze-thawing antigen and TNF-α, the percentageof CD1a、CD83、CD86wasincreased to 50.2±5.7, 60.5±16.5 and 56.2±16.4, respectively, (P0.01) . The percentage of CD8~+ T cells in TDLNC increased from 32.8±3.2 to 62.5±2.5 after co-cultured with DC-Ag (P0.01) . CONCLUSION: Typical DCs can be induced from the mononuclear cells in axillary draining lymph node after stimulated with cytokine (rhGM-CSF, rhIL-4 and TNF-α), which possess strong antigen presentation function and can stimulate TDLNC to proliferate and differentiate into antigen specific CTL.
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