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《Chinese Journal of Cellular and Molecular Immunology》 2010-01
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Effect of inducible nitric oxide synthase on pancreas islet apoptosis in rats

LI Bai-feng,LIU Yong-feng,CHENG Ying,ZHANG Jia-lin Department of General Surgery and Organ Transplantation,First Hospital,China Medical University,Shenyang 110001,China  
AIM:To investigate the effect and mechanism of cytokine and inducible nitric oxide synthase on apoptosis and function of rat pancreas islets cultured in vitro.METHODS:Islets from Wistar rats were cultured in vitro and divided randomly into four groups:blank control group,cytokine group of islets cultured with TNF-α+IL-1β,aminoguanidine(AG)group of islets cultured with aminoguanidine,and AG + cytokine group of islets cultured with TNF-α+IL-1β and aminoguanidine.The nutrient fluid nitric oxide level and islets cNOS/iNOS activity were detected by test kit and the expressions of iNOS mRNA and apoptosis related gene(Bax,Bcl-2)were evaluated by RT-PCR.The viability of the islets was examined by AO/EB staining and the function of the islets was detected by insulin secretion index assay.RESULTS:After co-cultured with cytokines IL-1β and TNF-α,the expression and activity of iNOS in islet tissues enhanced(38.93±4.72)U/mL and the concentration of NO in medium increased remarkably(313.0±35.4)mol/L.The survival rate of cells and the insulin secretion index decreased with the up-regulation of proapoptosis gene and down-regulation of anti-apoptosis gene.But the activity of cNOS remained unchanged.Aminoguanidine reduced the cell apoptosis and increased the survival rate and insulin secretion index,and the activity of iNOS was inhibited.CONCLUSION:iNOS plays an important role in the apoptosis of islets cultured by cytokines TNF-α and IL1-β.Aminoguanidine prevents the islets from the damage of iNOS,alleviates the impairment of cytokines to islets,lessens the cell apoptosis and ameliorates the survival and function of islets.
【Fund】: 公益性卫生行业科研专项重点项目(卫生部361200802003)
【CateGory Index】: R587.1
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