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《Chinese Journal of Cellular and Molecular Immunology》 2010-01
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Role of transcription factor T-bet and Eomes in IFN-γ secretion of different human T cell subsets

WANG Hong-tao,GE Xiao-song,XUE Zhu-ping,LI Bai-qing Department of Immunology,Bengbu Medical College,Bengbu 233030,China  
AIM:To investigate the effect of siRNAs specific for T-bet and Eomesodermin(Eomes)in interferon-γ(IFN-γ)production of different human T cell subsets.METHODS:Double-stranded small interfering RNA(siRNA)sequences specific for genes of T-bet and Eomes were chemically synthesized and transfected into anti-CD3 mAb activated αβ T cells and Mtb-Ag activated γδ T cells.CD4+,CD8+ T and γδ T cells were sorted by flow cytometry and the expressions of T-bet and Eomes gene mRNA were detected by RT-PCR technique.The changes of IFN-γ production were determined by flow cytometry.RESULTS:After two series of transfection,the siRNA-FAM+ cells were about 50% in the transfected cells.The IFN-γ+ cells in CD4+ T cells(50.20%)decreased in the cells trasnsfected with T-bet siRNA(18.46%)but no obvious decrease was observed in the cells trasnsfected with Eomes siRNA,whereas the IFN-γ+ cells in CD8+ T cells(76.51%)decreased in the cells transfected with Eomes siRNA(25.37%)and no obvious decrease was observed in the cells transfected with T-bet siRNA.However,the IFN-γ producing cells in γδ T cells(76.52%)decreased in the cells transfected with T-bet siRNA(56.57%)or with Eomes siRNA(42.53%).CONCLUSION:At the level of transcription factors,T-bet and Eomes are important transcription factors for the regulation of IFN-γ production in CD4+ and CD8+ T cells,respectively.Both T-bet and Eomes may simultaneously be involved in the regulation of the IFN-γ production of γδT cells.
【Fund】: 安徽省自然科学基金项目(050430603);; 安徽省教育厅省高校省级重点实验室重点科研项目(2004sys008)
【CateGory Index】: R392.11
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