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Effects of ginkgolide B on proliferation,phagocytosis,NO and ROS production of murine peritoneal macrophages in vitro

WANG Xiao-dong,ZENG Yao-ying,SONG Bing Institute of Tissue Transplantation and Immunology,College of Life Science and Technology,Jinan University,Guangzhou 510632,China  
AIM:To explore the potential immunomodulatory effects and related mechanisms of ginkgolide B(GB),a known potent antagonist of platelet-activating factor receptor,we investigated the proliferation,phagocytosis,NO and ROS production of macrophage.METHODS:After murine peritoneal macrophages(PMs) preparation,PMs were treated with different concentrations of GB before culture time and then activated by LPS.Drug toxicology and PM proliferation were measured by MTT assays.Fluorescent beads ingestion and flow cytometry were used to assess phagocytosis of LPS-activated PMs.Griess reagent system was used to determine the amount of LPS-induced NO production.H2DCFDA labeling and flow cytometry were used to trace ROS level of both rest and LPS-activated PMs.RESULTS:In a dose-dependent manner,GB(5,10,and 20 μmol/L) significantly suppressed the phagocytosis as well as NO and ROS production at 24 h and inhibited cell proliferation at 48 h after LPS stimulation.CONCLUSION:According to these interesting effects of GB on macrophage behaving and functioning,it' s quite reasonable to do further studies of GB as a nature occurring immunomodulator candidate.
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