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Quercetin affects leptin and its receptor in human gastric cancer MGC-803 cells and JAK-STAT pathway

QIN Yan,HE Li-ya,CHEN Yong,WANG Wen-yi,ZHAO Xue-hua,WU Mian-yun Institute of Cellular and Molecular Biology,College of Medical Science,Wuhan University of Science and Technology,Wuhan 430065,China  
AIM:Quercetin affects the expressions of leptin and its receptor in human gastric cancer MGC-803 cells and JAK-STAT pathway.METHODS:The cultured MGC-803 cells were divided into three groups:Control group:the cultured cells without quercetin,and Quercetin group:the cultured cells with quercetin(40 μmol/L),and AG490group:the cultured cells with AG490(40 μmol/L)The expressions of Leptin,Leptin receptor and P-STAT3 were detected in protein level by immunocytochemical and Western bloting method respectively.The expressions of Leptin,Leptin receptor were detected in mRNA level by RT-PCR method.MGC-803 cell cycle was arrest by flow cytometry(FCM);MGC-803 cell apoptosis ratio by apoptotic marker An-necxinV.RESULTS:The protein expression of Leptin,Leptin receptor,P-STAT3 and the the mRNA expression of Leptin and Leptin receptor were significantly increased(P0.05),compared with the control group.There was the rectilinear correlation relationship not only between Leptin and P-STAT3 protein(r=0.741,P0.05) but also between Leptin receptor and P-STAT3 protein(r=0.693,P0.05).FCM analysis showed that quercetin arrested MGC-803 cells at the G2/M phase,The ratio of apoptotic and necrosic cells increased with added quercetin concentration.CONCLUSION:Quercetin could inhibit the Proliferation of MGC-803 cells.It is probably relevant to the down-regulation the expressions of Leptin and Leptin receptor protein,Leptin mRNA and Leptin receptor mRNA by JAK-STAT pathway.
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