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The effect of human placenta-derived mesenchymal stem cells on cord blood CD8~+T cells activation,cellcycle and secretion of IL-17

WANG Guo-yan1,ZHANG Si-ying1,LI Guang-yun2,WANG Fei-fei1,DU Hai-bo3,LUAN Xi-ying1 1Department of Immunology,Binzhou Medical University,Yantai 264003;2Qianfoshan Hospital,Jinan 250014;3Department of Gynaecology and Obstetrics,Laishan First People' s Hospital,Yantai 264000,China  
AIM:To study the effect of human placenta-derived mesenchymal stem cells(hPMSCs) on cord blood CD8+T cell activation,cell cycle and secretion of IL-17,and to provide the theoretical basis for it application in the cell-based therapies.METHODS:hPMSCs were isolated from mature placenta by the method of digestion.Then hPMSCs were cultured,expanded in vitro,and were used in test after the third passage.CD8+T cells were sorted from cord blood with immunomagetic beads.FCM was used to analyze the expression of early activation phenotype,cell cycle of cord blood CD8+T cells and cytokine secretion.RESULTS:CD8+T cells stimulated by PHA in the presence of hPMSCs were arrested at G0/G1 phase.The expression of the early activation marker CD25 and CD69 of cord blood CD8+T cells was inhibited in the presence of hPMSCs.While,IL-17secretion of cord blood CD8+T cells stimulated by PMA was increased.CONCLUSION:hPMSCs can suppress the activation of cord blood CD8+T cells by altering T cell cycle;up-regulate the level of IL-17 secreted by cord blood CD8+T cells.
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