Full-Text Search:
Home|About CNKI|User Service|中文
Add to Favorite Get Latest Update

mTOR/p70s6k signaling pathway is involved in megakaryocyte polyploidization

YU Hui-ying,MA Dong-chu,LIN Di,SUN Ying-hui,TAO Shun-yan Department of Experimental Medicine,Northern Hospital,Shen-yang 110840,China  
AIM:To investigate the mechanisms of megakaryocyte polyploid cell cycle control.METHODS:The expression and phosphorylation of mTOR/p70s6k pathway proteins was detected by western blot.Double-labeling techniques were used to investigate in which of the phase of the polyploid cell cycle S6K1 at Thr421/Ser424 are phosphorylated.RESULTS:Nocodazole induced a relatively synchronized polyploidization in Dami cells.The expression of mTOR and the phosphorylation of mTOR at Ser2448 increased when Dami cells begin to progress from G1 to S-phase in cell cycle.Analysis of flow cytometry showed that phosphorylation of S6K1 at Thr421/Ser424 increased at G2/M-phase.CONCLUSION:mTOR/S6K1 pathway is involved in megakaryocyte polyploid cell cycle control.
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©CNKI All Rights Reserved