mTOR/p70s6k signaling pathway is involved in megakaryocyte polyploidization
YU Hui-ying,MA Dong-chu,LIN Di,SUN Ying-hui,TAO Shun-yan Department of Experimental Medicine,Northern Hospital,Shen-yang 110840,China
AIM:To investigate the mechanisms of megakaryocyte polyploid cell cycle control.METHODS:The expression and phosphorylation of mTOR/p70s6k pathway proteins was detected by western blot.Double-labeling techniques were used to investigate in which of the phase of the polyploid cell cycle S6K1 at Thr421/Ser424 are phosphorylated.RESULTS:Nocodazole induced a relatively synchronized polyploidization in Dami cells.The expression of mTOR and the phosphorylation of mTOR at Ser2448 increased when Dami cells begin to progress from G1 to S-phase in cell cycle.Analysis of flow cytometry showed that phosphorylation of S6K1 at Thr421/Ser424 increased at G2/M-phase.CONCLUSION:mTOR/S6K1 pathway is involved in megakaryocyte polyploid cell cycle control.
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