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The efficacy of enhancing murine zona pellucid 3 DNA vaccine immunized through intranasal vaccination by IL-15

LIU Fu-pei,CHANG Dan,YU Yan-kun,ZHANG Fu-chun,ZHANG Ai-lian Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,College of Life Science and Technology,Xinjiang University,Urumqi 830046,China  
AIM:To observe the efficacy of enhancing murine zona pellucid 3(mZP3) DNA vaccine immunized through intranasal vaccination by IL-15 as adjuvant and chitosan as delivery vactor.METHODS:C57BL/6 mice were delivered with chitosan-loaded mZP3 DNA vaccine or IL-15+pcD-mZP3 vaccine by intranasal route,and to detect specific IgG in serum and sIgA in vaginal fluid by indirect ELISA,and to perform fertility experiment that all female C57BL/6 mice were mated(one male for each female) with normal males of similar age.To do histology analysis of immunized mice ovary and lungs for morphology of ovary.RESULTS:The results of ELISA showed IL-15 can promote IgG antibody level of immunized mice,and the fertility rate was decreased to some degrees.CONCLUSION:IL-15 and chitosan can enhance the antibody levels induced by mZP3 DNA vaccine,and reduce murine fertility rate to a certain extent.
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