Full-Text Search:
Home|About CNKI|User Service|中文
Add to Favorite Get Latest Update

Effects and molecular mechanisms of inhibiting FOXM1 expression on ovarian cancer cell line SKOV3 by Lentiviral vector

CHEN Guo-qing,YAO Zhen-wei,QI Hong-bo,ZHANG Hua,LUO Yi,QIN Xing-fa Department of Obstetrics and Gynecology,the First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China  
AIM:To investigate the effects and possible molecular mechanisms of inhibiting FOXM1 expression on SKOV3 cells by lentiviral vector targeting FOXM1 shRNA.METHODS:SKOV3 cells were infected by lentiviral vector targeting FOXM1 shRNA with a multiplicity of infection(MOI) of 20,then growth curve of SKOV3 cells was determined by MTT assay,cell cycle was analysed by flow cytometry(FCM),and the expression of mRNA and protein of FOXM1,Cyclin D1,PLK1 by Real time PCR and Western blot.RESULTS:Lentiviral vector targeting FOXM1 shRNA with a multiplicity of infection(MOI) of 20 could significantly inhibit the growth of SKOV3 cells.After infected by lentiviral vector targeting FOXM1 shRNA,the G0/G1 phase cells increased and the S-phase cells decreased,and the expression of mRNA and protein of FOXM1,Cyclin D1,PLK1 of SKOV3 cells were significantly down-regulated.CONCLUSION:Inhibiting FOXM1 expression has a significantly effect of inhibiting proliferation on SKOV3 cells.Blocking SKOV3 cells in the G0/G1 phase by down-regulating the expression of Cyclin D1,PLK1 protein may be its mechanism.
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©CNKI All Rights Reserved