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Cloning and identification of the variable region gene of mouse anti-human BAFF mAb

XIAO Li-ming1,GONG Jiu-yu1,WANG Chang1,CHEN Heng-yue1,LI Xiang-dong2,JIN Bo-quan1,CHEN Li-hua1 1Department of Immunology,2Xijing Orthopedic Hospital,Xijing Hospital,the Fourth Military Medical University,Xi' an 710032,China  
AIM:To obtain the variable region gene sequence of heavy and light chain of mouse anti-human BAFF monoclonal antibody(mAb) on base of BAFF mAb which was cloned in our laboratory.METHODS:The total RNA was extracted from mouse anti-human BAFF mAb hybridoma cell line FMMUB4,and then the RNA was reverse transcribed into cDNA.Specific primers were designed to amplify the targeted gene.The targeted gene fragments were inserted into vectors to construct the clone vectors.The gene sequences were analyzed after identified by positive clones screening and restrictive enzyme digestion.RESULTS:The variable region gene sequences of mouse anti-human BAFF mAb were obtained.CONCLUSION:The variable region gene sequences of mouse anti-human BAFF mAb will provide experimental basis for further study on constructing engineered antibodies.
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