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《Chinese Journal of Cellular and Molecular Immunology》 2014-01
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Bone marrow-derived mesenchymal stem cells from estrogen deficiency induced osteoporosis mice regulate T cell migration and apoptosis through expressing MCP-1

YU Yang;SHAO Bingyi;SHANG Fengqing;ZHOU Zhifei;WANG Xi;SHUAI Yi;LIAO Li;JIN Yan;YANG Deqin;Department of Endodontics,Affiliated Hospital of Stomatology,Chongqing Medical University;Chongqing Key Laboratory of Oral Diseases and Biomedical Science,Chongqing Medical University;Department of Orthodontics,Fourth Military Medical University;Department of Pediatric Dentistry,Affiliated Hospital of Stomatology,Fourth Military Medical University;Department of Oral Histology and Pathology,School of Stomatology,Fourth Military Medical University;  
Objective To reveal the role of bone marrow-derived mesenchymal stem cells( BMSCs) in the development of osteoporosis by comparing the differences in monocyte chemoattractant protein-1( MCP-1) expression and T cells' migration and apoptosis induced by BMSCs from ovariectomy( OVX) group and sham group. Methods OVX was performed on C57BL /6 mice to establish the animal models of osteoporosis. Osteoporosis was confirmed by micro-CT. The expression of MCP-1 between OVX group and sham group was examined by ELISA; after exogenous estrogen of different concentrations were given to stimulate BMSCs from OVX group,the expression of MCP-1 was observed again by ELISA. Through co-culturing of BMSCs and T cells,the change of T cells' migration and apoptosis capacity induced by BMSCs was compared between OVX group and sham group. And also,we observed the effects of exogenous estrogen of different concentrations on the T cells' migration and apoptosis capacity. Results In animal models of osteoporosis induced by estrogen deficiency,BMSCs had a declined inducing effect on the capacity of T cell migration and apoptosis and expressed a decreased level of migration-related gene MCP-1. After the stimulation of estrogen of certain concentration,the declining tendency was revised to some extent. Conclusion Through expressing MCP-1,BMSCs could regulate the capacity of T cell migration and apoptosis,thus leading to the development of osteoporosis.
【Fund】: 国家重点基础研究发展计划(973)(2011CB964700);; 重庆市医学重点学科建设项目《牙体牙髓病学》(2011年)
【CateGory Index】: R580
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Chinese Journal Full-text Database 5 Hits
1 YU Yang;SHAO Bingyi;ZHOU Zhifei;SHANG Fengqing;SHUAI Yi;WANG Xi;LIAO Li;JIN Yan;YANG Deqin;Department of Endodontics,Affiliated Hospital of Stomatology,Chongqing Medical University;Chongqing Key Laboratory of Oral Diseases and Biomedical Science,Affiliated Hospital of Stomatology,Chongqing Medical University;Department of Pediatric Dentistry,Fourth Military Medical University;Department of Orthodontics,Affiliated Hospital of Stomatology,Fourth Military Medical University;Department of Oral Histology and Pathology,School of Stomatology,Fourth Military Medical University;;Role of bone marrow-derived mesenchymal stem cells in treating estrogen deficiency induced osteoporosis[J];Chinese Journal of Cellular and Molecular Immunology;2013-12
2 LIN Fengjin;WANG Shui-liang;HUANG Liang-hu;WANG Jin;ZHU Ling;WANG Qing-hua;TAN Jianming;Fujian Key Laboratory of Transplant Biology, Fuzhou General Hospital;;Establishment of technology of co-expression of green fluorescent protein and luciferase in human umbilical cord mesenchymal stem cell via lentiviral expression system[J];Chinese Journal of Cell and Stem Cell(Electronic Edition);2013-03
3 WANG Zhenheng,ZHAO Jianning,WANG Rui.Department of Orthopedics,Nanjing General Hospital of Nanjing Military Command,Nanjing 210002,China;Research progress in osteoporotic models[J];Chinese Journal of Osteoporosis;2012-07
4 SHAO Bingyi;YU Yang;SHUAI Yi;QI Meng;LIAO Li;JIN Yan;YANG Deqin;Department of Endodontics,Affiliated Hospital of Stomatology,College of Stomatology,Chongqing Medical University;Chongqing Key Laboratory of Oral Diseases and Biomedical Science,Chongqing Medical University;Tissue Engineering Center,Hospital of Stomatology,Fourth Military Medical University;;Estrogen promotes osteoclast apoptosis by inducing Fas ligand expression in bone marrow-derived mesenchymal stem cells in vitro[J];Chinese Journal of Cellular and Molecular Immunology;2013-10
5 YU Yang;SHAO Bingyi;SHUAI Yi;SHANG Fengqing;WANG Xi;ZHOU Zhifei;LIAO Li;JIN Yan;YANG Deqin;Department of Endodontics,Affliated Hospital of Stomatology,Chongqing Medical University;Chongqing Key Laboratory of Oral Diseases and Biomedical Science,Chongqing Medical University;Department of Oral Histology and Pathology,School of Stomalogy;Department of Orthodontics, Affliated Hospital of Stomalogy,Fourth Military Medical University;Department of Pediatric Dentistry,Affliated Hospital of Stomalogy,Fourth Military Medical University;;Role of bone marrow-derived mesenchymal stem cells in treating colitis through Fas /FasL-mediated immune regulation[J];Chinese Journal of Cellular and Molecular Immunology;2013-10
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