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《Chinese Journal of Cellular and Molecular Immunology》 2015-01
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Lentiviral vector-mediated Nanog short-hairpin RNA inhibits the growth of human gastric carcinoma xenograft in nude mice

LI Kewei;JIANG Zheng;Department of Gastroenterology,First Affiliated Hospital,Chongqing Medical University;  
Objective To construct lentiviral vector that interferes with Nanog,investigate its expression in human gastric cancer cell SGC-7901-transplanted nude mice,and explore the effect of shRNA-Nanog transfection on the growth of the xenograft in mice.Methods Lentivirus carrying shRNA-Nanog was prepared by incorporating previously validated siRNA into Nanog gene specific lentiviral vectors.The models of human gastric cancer transplantation were constructed in nude mice.The mouse models were randomly divided into lentivirus-shRNA-Nanog transfection group(experimental group),GFP infection group(empty vector group) and uninfected group(control group).The tumor volume and mass changes were measured.Small animal in vivo imaging was employed to examine the fluorescent intensity and tumor metastasis.The Nanog protein expression was determined by Western blot analysis.The apoptosis of transinfected tumor cells was analyzed by TUNEL method.Results The gene sequencing demonstrated that the recombinant lentivirus carrying shRNA-Nanog was successfully established.In vivo imaging showed that 27 days after transfection,the total fluorescent intensity in the experimental group was significantly lower than that in the empty vector group or the uninfected group.The xenograft volume and mass in the experimental group decreased significantly as compared with those in the empty vector group or the uninfected group.Western blotting indicated that the expression of Nanog protein in the experimental group was significantly lower than that in the empty vector or uninfected group.TUNEL results revealed that the apoptosis rate in the experimental group was significantly higher than that in the other two groups.No statistically significant difference was found between the empty vector group and the uninfected group.Conclusion We successfully established Nanog gene-shRNA expression vector and capsulated it as lentivirus particles,which could inhibit the growth of xenograft in nude mice.
【CateGory Index】: R735.2;R-332
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【Citations】
Chinese Journal Full-text Database 2 Hits
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【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 YAO Su-mei;HE Hai-yan;CHEN Jin-liang;Nantong First People's Hospital of Jiangsu Province;;Correlations of Nanog Expression in Lung Adenocarcinoma Tissue with Chemotherapy Response and Prognosis[J];医学临床研究;2016-12
2 Ning Yanyang;Li Jinling;Xu Qiong;Guanghua School of Stomatology,Hospital of Stomatology,Sun Yat-sen University,Guangdong Provincial Key Laboratory of Stomatology;;Effects of Nanog overexpression on the proliferation and multipotent differentiation potentiality of human dental pulp cells[J];中华口腔医学研究杂志(电子版);2015-05
3 Li Yang;Zhao Yong-fu;Department of Gastrointestinal Surgery,Second People's Hospital of Nanyang;First Affiliated Hospital of Zhengzhou University;;Stomach cancer stem cells:isolation, identification and characteristics[J];中国组织工程研究;2015-32
4 YANG Zheng-yu;LIU De-chun;Third Affiliated Hospital,Henan University of Science and Technology;;Expression and Significance of OCT4,SOX2 and NANOG in Gallbladder Carcinoma[J];河南科技大学学报(医学版);2015-01
5 LI Kewei;JIANG Zheng;Department of Gastroenterology,First Affiliated Hospital,Chongqing Medical University;;Lentiviral vector-mediated Nanog short-hairpin RNA inhibits the growth of human gastric carcinoma xenograft in nude mice[J];细胞与分子免疫学杂志;2015-01
6 Ai-Xi Sun;Chang-Jiang Liu;Zi-Qin Sun;Zhi Wei;Department of Gastroenterology, The General Hospital of Jinan Military Command;;NANOG: A promising target for digestive malignant tumors[J];World Journal of Gastroenterology;2014-36
7 LIU Jie;XU A-man;LU Zhen;YUAN Xiao;GUO Tao;WANG Li-kun;Department of General Surgery,the Fourth Affiliated Hospital of Anhui Medical University;;Expression of Nanog and Oct-4 in gastric cancer and precancerous lesions and its significance[J];临床与实验病理学杂志;2014-08
8 HUANG Yan-yan;PEI Xue-lian;LIU Yang;LI Qiu-xia;WANG Yu-ting;MU Xiao-ling;Key Laboratory of Xinjiang Endemic and Ethnic Disease School of Medicine,Shihezi University;;Expression of tumor stem cell markers in the tumor sphere from esophageal squamous cells carcinoma Eca109[J];解剖学报;2014-03
9 Peng Te;Wang Bingyun;Li Dongsheng;Chen Zhisheng;Chen Shengfeng;Ji Huiqin;Chen Jinding;College of Veterinary Medicine,South China Agricultural University;School of Life Science,Foshan University;;Construction and Screening of Recombinant Interference Vector of Pluripotency Associated Genes cNanog and cPouV in Chicken Embryonic Stem Cells[J];生物技术通报;2014-05
10 LI Dong-sheng;CHEN Zhi-sheng;WANG Bing-yun;PENG Te;CHEN Sheng-feng;JI Hui-qin;CHEN Jin-ding;College of Veterinary Medicine,South China Agricultural University;School of Life Science,Foshan University;;Expression and Localization of cPouVand cNanog Genes in the Chicken Embryo Pluripotent Cells Development[J];畜牧兽医学报;2014-05
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