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《Chinese Journal of Cellular and Molecular Immunology》 2015-01
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Activation of Wnt/β-catenin pathway in NK cells by glycogen synthase kinase-3β inhibitor TWS119 promotes the expression of CD62L

CHEN Yongqiang;ZHENG Lu;LIU Junquan;ZHOU Zhonghai;L Xiaoting;ZHANG Juan;ZHANG Song;SUN Leiqing;CHEN Fuxing;Department of Central Laboratory,No.97th Hospital of PLA;  
Objective To investigate the effect of Wnt/β-catenin pathway activation by glycogen synthase kinase-3βinhibitor 4,6-disubstituted pyrrolopyrimidine(TWS119) on proliferation and phenotypic characteristics of human nature killer(NK) cells.Methods The peripheral blood mononuclear cells(PBMCs) were obtained from healthy volunteers and added to the complete medium containing recombinant human interleukin-2(rh IL-2) and human AB serum to isolate NK cells from PBMCs.After co-cultured with 0-8.0 μmol/L TWS119 for 72 hours,growth curve and Wnt/β-catenin activation of NK cells in each group were determined by CCK-8 and Western blotting.The CD107 a and CD62L(L-selectin) expressions in the NK cells were detected using flow cytometry.Results NK cells were amplified to(61.76 ± 3.74) % after human PBMCs were cultured for 10 days.The 0-2.0 μmol/L TWS119 could promote the growth of NK cells in a dose-dependent manner,and the proliferation rate gradually dropped when TWS119 was more than 2.0 μmol/L.0-8.0 μmol/L TWS119 could activate Wnt/β-catenin pathway and up-regulate the expression of CD62 L in a dose-dependent manner,but it decreased the expression of CD107 a.Conclusion Human NK cells isolated from peripheral blood treated with TWS119 gave rise to early mature CD62L+NK cells.
【Fund】: 南京军区医学科技创新研究基金(11MA040)
【CateGory Index】: R73-36
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