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Detection of Cervical Cancer Antigen in Human Sera with Monoclonal Antibody AU_(14-1)-ELISA Assay

Hu Zhiwei; Wu Suyun and Wen Gansheng(Department of Tumor Immunology, Jiangxi Medical College, Nanchang 330006)  
The monoclonal antibody against mouse cervical cancer,designated AU14-1,was conjugatedto horseradish Peroxidase(HRP)and a sandwich ELISA with AU14-1 and HRP-AUI14-1 conjugatewas established. By this immunoassay, positive resultS were found in the 25 PatientS with cervical cancer including 19 cases with squamous cell carcinoma,4 adenocarcinoma,I clear cell carcionma and 1malignant mixed mesomerdal), in none of the 40 normal females, 2 of the 52 PatientS with cervicitis andnone of the 12 cases of other gynecological diseases.In addition,one of bloody ascites from a Patientwith ovarian cancer was also negative.It showed that the sandwich ELISA With McAb AU14--lwe usedhad better sensitivity and speCificity than those of assays for other serum markers(SCC and CA--125)of cervical carcionma and it appeared promising for clinical application.It also further verified the theory of "evoluhon antigen"in tumor.
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