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Preparation of Monoclonal Antibodies of Calcitonin Gene Related Peptide(CGRP)and Their Perliminary Characterization

Lu Qing,et al(Zhujiang Hospital,lst Milinary Medical University,Guangzhou 510280)  
An coupling complex of CGRP-BSA was used to immunize BALB/c mice via intra--spleenand three hybridoma cell lines, 3B4, 7E11 and 8F2, were obtained by fusing SpZ/0 myeloma cell lineswith the complex-primed mouse spleen cells.By double gel diffusion test, 3B4 was shown to be a lgGI,while both 7E11 and 8F2 were IgM.The number of chromosomes of hybridomas were betwen 92 to114.The McAb titers of 8F2,3B4 and 7E11 ascites is 1.5×10-5,3.1×10-5 and 1.25×10-4, respectively.Under anesthesia, the right carotid artery of experimental Wistar ratS was cannulated withPOly-ethyene tube connected to a pressure transducer for recording mean arterial pressure on aPOlygraph.When CGRP alone infuses into the rats(3ng/0.1ml each rat),the BP-decreasing value is a6.665~9.335kpa,while CGRP(3pg) plus McAb 8F2(1: 100)are administrated value of BP is 11.997kpa,2.665~5.332kpa lower than only infusion of CGRP It is suggested that binding of 8F2 to its corresponding antigen lead to futher exPOsure of CGRP's domain and so enhance the depressor resPOnse toCGRP.
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