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One-Step Purification of Monoclonal Antibody on Preparative Scale by Gel Filtration'Chromatography

Liu Li; Liu ZhiGuang; Liu Cheng gang;Chen Zhinan; Sui Yanfang and Xu Liqing(Department of pathology, Fourth Military Medical University, Xian 710032)  
One-step method for purificative of McAb on a preparation acale has been established byfast protein liquid chromatography(FPLC)using Pharmacia sephacryl S-300 gel. Anti-hepatomaMcAb HAb 18 ascites was directly purified on a gel filtration chromatography and eluted by PH7.410 mmol/L PBS at 0.5 ml/ min each time, the sample loaded was 40-50 ml of ascites and about400 mg of IgG could be in an operating cycle of 4 hours.The yield of IgG was 8.5-9 mg/ml asoitesfluid and the recovery rate was 85 %~90%.The purity of McAb was about 90% by SDS--PAGE andthe formunoactivity was l:80000(7.8×10-10 mol/ L)by ABC staining.
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