Amplification of Immunoglobulin Genes from Human Peripheral Blood Lymphocytes by PCR
Wan Zesheng, et al.(Department of Microbiology,The Third Military Medical University, Chongqing 630038)
Employing routine PCR and half nested PCR, the genes of human immunoglobulin Fd and light chains were amplified from human peripheral blood lymphocytes by sets of oligonucleotide primers designed by Kang. Some primers could not directly amplify antibody genes from cDNA templates. So, a hald nested PCR method was used with primers designed to maximize homology with human immunoglobulin signal peptide sequences as the first round PCR. The molecular weight of products amplified by routine and half nested PCR is identical to that reported by other scholars. These results suggest that half nested PCR might be helpful for maximizing the diversity of heavy and light chain genes of human immunoglobulin.