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Construction and Analysis of Three dimensional Graphic Model of Single Chain Fv Derived from an Anti HSV Monoclonal Antibody by Computer

Yang Qiaoxin, Ma Wenyu, et al.(Department of Microbiology, Fourth Military Medical University, Xi’an 710032)  
A three dimensional graphic model of an adulterated ScFv, of which the heavy chain obtained from an anti Herpes simplex virus(HSV) McAb and the light chain from an anti Hemorrhagic fever with renal syndyome virus(HFRSV) McAb, was constructed by using a homologus protein pridecting computer algorithm at SGI graphic computer station. We investigated the structure, static electricity and hydrophobicity of ScFv fragment. Computer graphic modelling indicated that all places of the linker, V L and V H were suitable. The light chain, as well as the heavy chain, was involved in composing the “hydrophobic pocket”, and took the two fifths of the “hydrophobic pocket” mouth.The linker was isolated from V H and V L. CDRs of V L and V H sorrounded “hydrophobic pocket”. This study provides a method for improving antibody affinity, investigating antibody structure and analyzing function of light, as well as heavy chain in antibody activity.
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