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《Journal of Northwest Sci-Tech University of Agriculture and Forestry》 1989-01
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INDUCING OF ENDOMITOSIS AND ENDOREDUPLICATION OF DON CELLS

Zhan Tiesheng (Department of Animal Science, Northwestern Agricultural University}K. L.Satya-prakash {Department of Genetics,the University of Texas M.D.Anderson Hospital andTumor Institute,at Houston)  
In the research, the Don cells were treated with DES(diethyl stilbestrol) to induce endomitosis and endoreduplication using different dosages of DES and time of treating and recovering.Good result was obtained in the test , that 56.3% of the cells in mitosis was shown as polyploid and endoredupliceating cells in one of the test groups; 36,5% of endoreduplicating cells in another group.The percentage of induced cells was 2,4 times higher than that by Jackson, and it was also found that DES was a good inducing agent to obtain endomitosis and endoreduolication whose toxicity was low to the cells and large in the differences between the effective dosage and poisonous dosage of the chemicals using to induce endomitosis and endoreduplication.In the range of effective dosage the highest was 10 times as high as the lowest.
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