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Experiments on in Vitro Capacitation, of Ejaculated Sperm and in Vitro Fertilization in Rabbit

Xu Jun Qian Jufen Zhang Yong Wang Jianchen {Department of Veterinary Medicine) . , . .  
The purpose of this research was to establish an available simplified method that would enable ejaculated rabbit sperm to coplete in vitro capacitation and obtain in vitro fertilization, early cleavage and normal offspring of rabbit ova. In vitro capacitation of sperm was carried out by ; using three methods. A. in high,, ;iprue strength solution followed by prein-cubation in defined medium for 1-2 hours) B.in HIS followed by preincu-bation in DM for 11-12 hours C. in m-HIS ( 10 mL m-DM + 34 mg NaCl) followed by preincubation in m-DM (100 mL DM + 84mg NaHCO3) for 2-4 hours. Five hundred and twelve oocytes were cocultured in DM or m-DM with above three kinds of capacitated sperms.The fertilization and early cleavage were retarded in group Aj the fertilization ova almost stayed in pronucleus period in group Bj good result of fertilization and early cleavage were obtained in group C. Sixty 2-4 cell embryos from group C were transferred into nine recipients, five of which became pregnant. Four embryos were carried successfully to deliver normal offspring by two reci-i. i , pients.. Laparotomies were, performed in-three recipients to assess Implantationabout two weeks after embryo transfer, and twelve emhryos were implanted.
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