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《Journal of Northwest A & F University(Natural Science Edition)》 2011-01
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Cloning and analysis of the complete genome of CSFV isolate SXCDK from Shaanxi province

LI Ling-wei1,2,ZHANG Zhi2,WU Fa-xing2,CHENG Wei1,2,YANG Ruo-song1,2,ZHANG Yan-xia2,LIU Shuang2,ZHENG Hui2,LI Xiao-cheng2,CHEN De-kun1(1 College of Veterinary Medicine,Northwest A&F University,Yangling,Shaanxi 712100,China;2 China Animal Health and Epidemiology Center,Qingdao,Shandong 266032,China)  
【Objective】 The study was to isolate CSFV strain SXCDK,determine the whole genome sequence,study its molecular characteristics and the relationship with other strains,accumulated research data for molecular epidemiology of classical swine fever epidemic strain virus.【Method】 The CSFV strain SXCDK was obtained after virus isolation from a suspected patholgical material of CSF collected from Xi'an,Shaanxi province.According to the genome sequences of classical swine fever virus(CSFV) published in GenBank and the reference,eleven pairs of specific primers were synthesized.By using RT-PCT,the genome of a CSFV isolate SXCDK was gained after eleven fragments had been successfully amplified(GenBank accession:GQ923951).Genome comparison of the SXCDK isolate with other known CSFV isolates was performed and analyzed by using software such as DNAstar,ClustalX 1.83 and Mega 4.1.【Result】 The genome of a CSFV isolate SXCDK,isolated from Shaanxi province,is 12 296 nt in length.It is composed of a 373 nt 5′terminal untranslated region(UTR),a 11 697 nt open reading frame(ORF) encoding a polyprotein of 3 899 amino acids(aa),and a 226 nt 3′UTR.SXCDK and other reported strains share 83.6%-96.2% identity at the nucleotide level and 91.3%-97.8% identity at the amino acid level.From an evolutionary point of view,isolate SXCDK is closely related to Taiwan isolate 96TD,with a pairwise distance of 0.038,distantly related to the isolate SXYL2006,which is also isolated from Shaanxi province,with pairwise distance 0.065.【Conclusion】 Isolate SXCDK is the first CSFV subgroup 2.1a strain whose genome sequence has been determined in the mainland of China,has the highest genetic relationship with 96TD,which also belongs to this subgroup.
【Fund】: 农业部动物疫情防治与监测项目(NY200609-03)
【CateGory Index】: S852.651
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