Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Northwest A & F University(Natural Science Edition)》 2011-01
Add to Favorite Get Latest Update

Enzyme analysis,gene CDs cloning and expression in E.coli of Mulberry Borer(Apriona germari hope) endogen β-1,4-endoglucanase

XU Wei-jia1,HAN Wei-jie1,LI Wang1,2,CHEN Yu-lin1(1 College of Animal Science and Technology,Northwest A&F University,Yangling,Shaanxi 712100,China;2 College of Animal Science and Technology,Henan Science and Technology University,Luoyang,Henan 471003,China)  
【Objective】 This objective focused on endogen β-1,4-endoglucanase gene of mulberrying borer,with the purpose of supplying the theory basis for constructing engineering bacteria which can decompose cellulose effectively.【Method】 The activities of endogen β-1,4-endoglucanase were measured by 1% of CMC-Na,total RNA was isolated from Mucberry Borer gut by trizol,the first cDNA chian was used as template,a pair of primers was designed based on the gene whose accession number is AY771358.1,for PCR endogen β-1,4-endoglucanase gene of Mulberry Borer,DNASTAR and BLAST of NCBI were used for sequence analysis;another pair of primers was designed based on the sequence of this study,which was to add restriction enzyme cutting site BamHⅠfor up site and SacⅠfor down site,PCR endogen β-1,4-endoglucanase gene of Mulberry Borer,and structure pET-APEG for prokaryotic expression in Escherichia coli BL21(DE3).【Result】 The most suitable pH range is from 4.4 to 5.6,with peak at 5.0;the most suitable range temperature is from 30 to 50 ℃,with peak at 50 ℃.If solution of endoglucanase is treated in water bath of 37 ℃ for 30 min,the activities hardly change;However,after treated in water bath of 60 ℃ for 30 min,the activities plummet.The length of CDs of β-1 and 4-endoglucanase is 978 bp,ecoding 325 amino acid residues;The result of BLAST showed that,the sequence identity of this gene is 85% compared with the cellulase gene of yellow-spotted longicorn beetle,Psacothea hilaris which belongs to the GHF5,and the sequence identity of protein reaches 90%,the sequence identity of this gene is 98% compared with the sequence of Ag-EaseⅢ which has been reported in mulberry borer,the sequence identity of protein reaches 99%.Structed pET-APEG for expression in E.coil BL21(DE3) and SDS-PAGE result showed,pET-APEG vector was expressed as about 55 ku polypeptide in E.coli BL21(DE3).【Conclusion】 The mulberry borer of Shannan area belongs to GHF5,and has multi-domains of endogen β-1,4-endoglucanase,and it's gene can be expressed in E.coil BL21(DE3).
【Fund】: 农业部现代农业产业技术体系建设专项(nycytx-40)
【CateGory Index】: Q786
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 9 Hits
2 Yin Youping; Cheng Jingqiu; Jiang Shunan (Southwest Agricultural University Chongqing 630716);STUDY ON THE KINETIC PROPERTIES OF CELLULASE IN APRIONA GERMARI HOPE (COLEOPTERA: CERAMBYCIDAE)[J];SCIENTIA SILVAE SINICAE;1996-05
3 SUO Feng-mei~1,LIN Chang-chun~1,WANG Hao-jie~1,DING Zhong-wen~2,XU Tian-sen~1(1.Research Institute of Subtropical Forestry,CAF,Fuyang311400,Zhejiang,China; 2.Fuyang Forestry Bureau, Zhejiang Province,Fuyang311400,Zhejiang,China);Study on the Character of Cellulase in Monochamus alternatus[J];Forest Research;2004-05
4 SUO Feng-mei1,2,WANG Hao-jie1,DING Zhong-wen3,XU Tian-sen1 (1.Research Institute of Subtropical Forestry,CAF,Fuyang 311400,Zhejiang,China;2.Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Chinese Peking Union Medical College,Beijing 100094,China;3.Forestry Bureau of Fuyang City,Zhejiang Province,Fuyang 311400,Zhejiang,China);Study on the Character of Cellulase in Monochamus alternatus Ⅳ.Effect of Feeding Conditions on the Monochamus alternatus Cellulase Activity[J];Forest Research;2007-03
5 CHANG Qiao-ling, SUN Jian-yi*, XU Ying-lei, QIAN Li-chun (Key Laboratory of Animal Molecular Nutrition,Ministry of Education,Feed Science Institute,Zhejiang University,Hangzhou 310029,China);Expression of A Thermostable Endoglucanase Gene celD from Clostridium thermocellum in Escherichia coli and Characterization of Its Recombinant Enzyme[J];Journal of Agricultural Biotechnology;2006-06
6 HUANG Yan,LING Min,QIN Yong-ling,LIANG Zhi-qun(College of Life Science and Technology,Guangxi University,Nanning 530004,China);Cloning and Expression of EndoglucanaseⅠ of T.Knoningii[J];Biotechnology;2008-02
7 Xu Jie,Yang Qian (Department of Life Science and Engineering,Harbin Institute of Technology,Harbin 150001,China);A STUDY ON CELLULASE PRODUCTION WITH AN EXCELLENT STRAIN STREPTOMYCES SP. C-5[J];Acta Energiae Solaris Sinica;2009-05
8 LU Jing-lin,HUANG Ai-ling,ZHENG Rong,LI Dian-dian,LU Tun(College of Biological Science and Engineering,Fuzhou University,Fuzhou 350108,China);Screening,Enzyme-producing Conditions Optimized and the 16S rDNA Sequences Analyzed of a Cellulose Decomposing Bacteria[J];Biotechnology;2009-06
9 DUAN Xu1,ZHANG Jing-jing1,ZHU Jing1,CAI Hu-hua2,CHEN Fen-fen1,GUO Ai-wei1(1.Faculty of Conservation Biology,Southwest Forestry College,Kunming 650224,China;2.ASAHI CHEMICAL MFG.CO.,LTD.,Nara 636-0104 500,Japan);Comparison Research on Cellulase System from Monochamus alternatus and Cipangopaludina chinensis[J];Journal of Yunnan Agricultural University;2009-04
Chinese Journal Full-text Database 10 Hits
1 YANG Gui-jun et al(School of Life Science,Ningxia University,Yinchuan,Ningxia 750021);Distribution of Celluloses in Eucryporrhynchus chinenis(Olivier) and Effects of Metal Ions on Their Activities[J];Journal of Anhui Agricultural Sciences;2008-12
2 Luo Youqing\ Li Jianguang (College of Forest Resources and Environment, Beijing For. Univ., 100083, P. R. China );Strategy on Applied Technology and Basic Studies of Poplar Longhorned Beetle Management[J];JOURNAL OF BEIJING FORESTRY UNIVERSITY;1999-04
3 LI Xiao-juan1, YAN Xiong-fei1, LUO You-qing1*, TIAN Gui-fang2, SUN Hong21Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing Forestry University, Beijing 100083, P. R. China 2Horticulture Administration Bureau of Wuzhong City, Ningxia Province, Wuzhong 751100, P. R. China;Cellulase in Anoplophora glabripennis adults emerging from different host tree species[J];中国林学(英文版);2008-01
4 WANG Guo-hong,GENG Jun-li,HUANG Xiang-cai (College of Life Sciences,Fujian Normal University,Fuzhou 350108,China);Effects of 9 Kinds of Metal Ions on Cellulase Activity of Tenebrio molitor L.and the Growth of Larvae[J];Journal of Fujian Normal University(Natural Science Edition);2009-03
5 LI Xiao-juan1,YAN Xiong-fei2,LUO You-qing1,TIAN Gui-fang3,NIAN Yin-jie3,SUN Hong3 1 Key Laboratory for Silviculture and Conservation of the Ministry of Education,Beijing Forestry University,Beijing 100083,P.R.China 2 Yulin University,Yulin 719000,P.R.China 3 Forest Department of Wuzhong City,Ningxia Province,Wuzhong 751100,P.R.China;Cellulase in Anoplophora glabripennis adults fed on original host tree species and non-original host trees[J];中国林学(英文版);2010-01
6 CHI Ya-qin ZHU Jing DUAN Xu NIE Heng(Faculty of Conservation,Southwest Forestry University,Kunming Yunnan 650224);Analysis on Characterization and Constitution of Cellulase in Bess beetle[J];Modern Agricultural Sciences and Technology;2011-03
7 WANG Wen-kai(Department of Agronomy, Hubei Agricultural College,Jingzhou,Hubei 434025,China);Advances in Systematics on Cerambycidae[J];Journal of Hubei Agricultural College;2002-02
8 HUANG Xiang-cai,WANG Guo-hong(College of Life Sciences,Fujian Normal University,Fuzhou 350108,China);Study on the Character of Cellulase in Tenebrio molitor L.[J];Jiangxi Plant Protection;2008-02
9 LIU Li , WANG Zhong-Kang, YU He-Wei, MU Dong-Dong, YUAN Qing, YIN You-Ping(College of Bioengineering of Chongqing University, Genetic Engineering Research Centre of Chongqing University, Key Laboratory of Gene Function and Regulation at Chongqing, Chongqing 400030, China );Effects of feeding on tetracycline and streptomycin on growth and gut digestive enzymes of Hepjalus gonggaensis larvae[J];Chinese Bulletin of Entomology;2008-02
10 YANG Jian-Xia1,HAO De-Jun2,DAI Hua-Guo1(1.Department of Plant Protection Nanjing Agricultural University,Nanjing 210095,China;2.College of Forestry Resources and Environment Nanjing Forestry University,Nanjing 210037,China);Effect of different host plants on activitities of several enzymes of the larvae of Monochamus alternatus.[J];Chinese Bulletin of Entomology;2008-06
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 YUE Si-jun et al (College of Life Science,Ningxia University,Yinchuan,Ningxia 750021);Study on Isolation and Screening for Cellulose-decomposing Bacterial with High Cellulase Activity[J];Journal of Anhui Agricultural Sciences;2009-01
3 ZENG Fan jiang 1,2 ZHANG Xi ming 1 LI Xiao ming 1 (1 Xinjiang Institute of Ecology and Geography, CAS, Urumqi, 830011;2 Shenyang Institute of Applied Ecology, CAS, Shenyang 110016)$$$$;STUDY ON THE CHARACTERISTICS OF ALHAGI AND ITS IMPACT ON RESOVRCE PROTECTION AND DEVELOPMENT[J];Arid Land Geography;2002-03
4 FAN Jun, HAO Ming de (Institute of Soil and Water Conservation, Northwest Sci Tech University of Agriculture and Forestry, Yangling, Shaanxi 712100, China);Kinetic characteristics of urease and alkaline phosphatase in dry land farming[J];Agricultural Reseach In The Arid Areas;2002-01
5 PAN Jian-mei(School of Marine Science & Technology,Huaihai Institute of Technology,Lianyungang 222005,China);Study on the Screening of a Cellulase-producing Bacterium Strain and Its Enzyme-producing Conditions[J];Journal of Huaihai Institute of Technology(Natural Sciences Edition);2008-01
6 Xi Beidou 1 Liu Hongliang 2 Bai Qingzhong 1 Huang Guohe 3 Zeng Guangming 3 Li Yingjun 1 (1. Department of Environmental Engineering, Tsinghua University, Beijing 100084; 2. Chinese Research Academy of Environmental Science, Beijing 100012;;Study on current status of lignin and cellulose biodegradation in composting process[J];Technigues and Equipment For Enviro.poll.cont;2002-03
7 Yang Qian  , Kasem Soytong  . Harbin Industrial University, Life Science Department,150001 Harbin;The Biological Degradation of Perishables in Urban Domestic Waste.[J];ENVIRONMENTAL SANITATION ENGINEERING;2000-03
8 YANG Yedong 1,HANG Yijun 2,ZHANG Jinlong 3 (1 China Academic Science and Development,Zhejiang Branch,Hangzhou 310012 Zhejiang,China; 2 Mudanjiang zhelong Medicine Scientific CO Led, Mudanjiang 157013 Heilongjiang,China; 3 Wenxhou Jun'er Print;Research,Application and Production of Cellulase in Weave Industry[J];ACTA LASER BIOLOGY SINICA;2000-02
9 WANG Tian-Hong; WU jing; ZOU Yu-Xia(State Key Laboratory of Microbial Technology, Chandong University. Jinan 250100);ADVANCES OF MOLECULAR BIOLOGY OF TRICHODERMA REESEI[J];MYCOSYSTEMA;2000-01
10 Zhu Ling-Xiang Yu Wei Liang Gai-Qin Dong Zhi-Yang (Institute of microbiology, Chinese Academy of Sciences, Beijing 100080);CLONE AND SEQUENCE ANALYSIS OF THE CELLOBIOHYDROLASE II GENE (CBH2) FROM TRICHODERMA KNINGII K801[J];Mycosystema;2001-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved