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《Journal of Northwest A & F University(Natural Science Edition)》 2011-01
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Antimicrobial Inhibition of polyhydroxy dinaphthaldehyde extracts(WCT) on Pseudomonas syringae pv.actinidiae in kiwifruit

WEI Hai-juan,LIU Ping,YANG Yan,WU Yun-feng(College of Plant Protection,Shaanxi Key Laboratory of Molecular Biology for Agriculture,Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education,Northwest A&F University,Yangling,Shaanxi 712100,China)  
【Objective】 The research was conducted to explore the antibacterial activity of WCT extracts of polyhydroxy dinaphthaldehyde against Pseudomonas syingae pv.actinidiae.【Method】 With inhibition zone method and and kiwifruit seedling inoculated with P.syingae pv.actinidiae method to detect the inhibition effect of WCT on the growth of P.syingae pv.actinidia,a field experiment was conducted on 7-year-old kiwi trees.Polyphenol oxidase(PPO),peroxidase(POD)and pathogenesis-related protein activities induced by inoculation with WCT and P.syingae pv.actinidiae on kiwifruit were studied.【Result】 The EC50 value of WCT for P.syingae pv.actinidiae was 11.5 mg/mL.The activities of PPO and POD in kiwifruit increased siginificantly when inoculated with WCT and P.syingae pv.actinidiae and maintained at a high level throughout the experiment,in which WCT prevention activity of PPO and POD was higher than that of other treatments.Both WCT and P.syingae pv.actinidiae inoculation could increase the expression of a 35 ku protein in kiwifruit leaves.【Conclusion】 WCT had a significant inhibition on P.syingae pv.actinidiae in petri dishes and field,and WCT induced the host producing resistance,hence reducing disease incidence.
【Fund】: 公益性行业科研专项“生物源农药创制与技术集成及产业化开发”(200903052);; 陕西省农业攻关项目“陕西主要果树病害发生规律及控制技术研究”(2010K01-11);; 陕西省农业创新项目“猕猴桃细菌性溃疡病发生规律和防治研究”(2010NKC-08);; 高等学校学科创新引智计划项目(B07049)
【CateGory Index】: S436.634
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