Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Northwest A & F University(Natural Science Edition)》 2011-01
Add to Favorite Get Latest Update

Isolation,identification and growth conditions of endophytic fungi of Eucommia ulmoides Oliv. for production of PDG

LIU Chao1,SHI Jun-ling1,ZHOU Xiao-juan1,YANG Bao-wei1,DOU Xing-xia2(1 College of Food Science and Engineering,Northwest A&F University,Yangling,Shaanxi 712100,China;2 Linyi Institute of Product Quality Supervision & Inspection,Shandong Province,Linyi,Shandong 276001,China)  
【Objective】 The study aimed at isolating pinoresinol diglucoside(PDG)-producing endophytic fungi from Eucommia ulmoides Oliv.to identify the strain with the highest yield of PDG and to investigate the basic conditions for its growth.【Method】 Endophytic fungi were isolated from the surface sterilized bark of Eucommia ulmoides Oliv.and then,cultivated in liquid broth at flask level to test the PDG production.The strain with the highest PDG production was selected and identified based on the morphological characteristics and the sequence of rDNA in the ITS district.Basic growth conditions of the strain were also studied in aspects of temperature,pH,carbon source and nitrogen source by the single-factor experimental design.【Result】 Eighteen strains of endophytic fungi were isolated from the bark of Eucommia ulmoides Oliv.Only three of them had the ability to produce of PDG.The strain XP-8,with the highest PDG production of 11.65 mg/L,was identified as Phomopsis sp.It could grow in a wide range of conditions from 20 ℃ to 35 ℃ and pH 4 to 8 with the best growth at 28 ℃ and pH 5.The strain XP-8 could use glucose and maltose,but not malic acid,among the tested carbon sources.Sodium nitrate showed to be the most favorable one among the tested nitrogen sources,whereas aspartic acid could not be used at all.【Conclusion】 The PDG-producing endophytic fungi were isolated from Eucommia ulmoides Oliv..The best strain having the highest PDG production was identified,and the basic conditions for its growth were also studied.
【Fund】: 陕西省科技攻关项目(2008K02-09);; 全国大学生科技创新项目(G07036)
【CateGory Index】: S567.19
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 8 Hits
1 Wang Wei, Shi Junling, Yang Baowei (College of Food Science and Engineering, Northwest Agriculture and Forestry University, Yangling City, Shaanxi 712100, China);Optimization of conditions for production of pinoresinol diglucoside by a strain of Phoma sp.[J];Transactions of the Chinese Society of Agricultural Engineering;2008-06
2 Lin Fucheng Liu Xiaohong Wang Hongkai Zhang Chulong ( 1Life Sciences College of Zhejiang University, 2Biotechnology Institute of Zhejiang University, Hangzhou 310029,China);Recent Research and Prospect on Taxol and Its Producing Fungi[J];Acta Microbiologica Sinica;2003-04
3 FENG Guang-hui1,YU Hua-shun2,YAO Juan2,CHEN Ming-li1,ZHANG Zhi-xin1,GUO Ai-guang1(1 College of Life Sciences,Northwest A & F University,Yangling,Shaanxi 712100,China;2 Research & Development Center,The Angel Yeast Co.ltd,Yichang,Hubei 443003,China);Phylogenetic reclassification analysis of S. cerevisiae based on the internal transcribed spacer 1(ITS-1) sequence of rDNA[J];Journal of Northwest A & F University(Natural Science Edition);2007-11
4 LIU Chao,SHI Jun-ling,WANG Wei,ZHOU Xiao-juan(College of Food Science and Engineering,Northwest A&F University,Yangling,Shaanxi 712100,China);Optimization of nutrient elements for the production of pinoresinol diglucoside by a strain of Phoma sp. of Eucommia ulmiodes Oliv. using response surface analysis[J];Journal of Northwest A & F University(Natural Science Edition);2009-07
5 JIANG Xin-yi,YANG Zhong-lin,LI Ping(Key Lab of Modern Traditional Chinese Medicine Affiliated to Ministry of Education,China Pharmaceutical University,Nanjing 210038,China);Optimization of Processing Method for Determination of Pinoresinol Diglucoside in Eucommia ulmoides[J];Progress in Pharmaceutical Sciences;2009-01
6 WANG Ya-qin1,ZHANG Kang- jian2 (1.College of Food and Bioengineering,South China U niversity of Technology,Guangzhou 510640 ,China; 2 .Northwest Science and Technology U niversity of Agriculture and Forestry,Yangling712 100,China);Advances in research on secondary metabolites of Eucommia ulmoides[J];Chinese Traditional and Herbal Drugs;2004-07
7 CHEN Xiaoqing1, LI Yuping1, PENG Mijun1, WANG Yulin1, DEYAMA Takeshi2(1.College of Chemistry and Chemical Engineering, Central South University, Changsha 410083,China;2.Central Research Laboratories ,Yomeishu Seizo Co Ltd, Nagano 3994601, Japan);The extraction and purification of pinoresinol diglucoside in eucommia ulmoide oliver[J];Journal of Central South University of Technology(Natural Science);2003-03
8 JIANG Xian-quan1 WANG Wei1 TANG Jian-hui1 GUO Yu-ren2(1.Institute of Marine Bioprocess Engineering, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; 2.Shanghai Agro-technical Extension and Service Center, Shanghai 201103, China);Characteristics and biocontrol of Phomopsis rot on postharvest rockmelon[J];Acta Phytophylacica Sinica;2007-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 CHANG Ming-jin et al(Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agriculture Science,Haikou,Hainan 571101);Research and Development of Natural Medicine Taxol[J];Journal of Anhui Agricultural Sciences;2006-02
2 CHEN Li-jun et al (Xinyang Agricultural College,Xinyang,Henan 464000);Recent Research Advance in Endophytic Fungi of Medicinal Plant[J];Journal of Anhui Agricultural Sciences;2006-11
3 LIU Liu-jun~1;SHAN Chao~1;CHEN Su-wen~1;LI Chang-qiong~2.1 College of Materials Science and Technology, Beijing Forestry University, 100083, P. R. China;2 Chengdu Seventh People's Hospital, 610000, P. R. China.;Technological route of comprehensive utilization of leaves of Eucommia ulmoids.[J];Journal of Beijing Forestry University;2005-05
4 ZHANG Ying, ZHANG Wei - qiang; WANG Shu - min (Changchun University of Traditional Chinese Medicine,Changchun 130117,China; Hospital of WeihaiElconomic - Technological Development Area,Weihai 264200, China);The Study of Processing Ecommia ulmoides OLliv. by Salty Water[J];Journal of Changchun University of Traditional Chinese Medicine;2007-03
5 Zhao Dan 1,2 Zhao Degang 1,2,3 Li Yan 1,2 1 Guizhou Key Laboratory of Agro-Bioengineering, Guiyang, 550025; 2 The Laboratory of Gene engineering, College of Life Sciences, Guizhou University, Guiyang, 550025; 3 KeyLaboratoryofGreen Pesticide and Agricultural Biological Engineering MinistryofEducation, Guiyang, 550025;Studies on Construction of EuFPS Gene Plant Expression Vector and Agrobacterium-Mediated Genetic Transformation of Eucommia ulmoides Oliv.[J];Genomics and Applied Biology;2009-01
6 CHAI Yi-feng*,ZHU Zhen-yu and CHEN Xiao-fei(Department of Pharmaceutical Analysis,School of Pharmacy,Second Military Medical University,Shanghai 200433),Fenxi Shiyanshi,2010,29(9):78~122;Pharmaceutical analysis(I)[J];Chinese Journal of Analysis Laboratory;2010-09
7 DAI Wenliang,TAO Wenyi(Key Laboratory of Industrial Biotechnology,Ministry of Education,Jiangnan University,Wuxi 214122,Jiangsu,China);Preliminary study on fermentation conditions of taxol-producing endophytic fungus[J];Chemical Industry and Engineering Progress;2008-06
8 Liu Na,Chen Xiaoqing*,Du Hui,Xue Xiaojuan, Ouyang Dongsheng#(College of Chemistry and Chemical Engineering, Central South University, Changsha 410083; #Institute of Clinical Pharmacology,Central South University,Changsha 410078);Separation and Purification of the Liganas from Eucommia Ulmoide Oliver[J];Chemistry;2006-04
9 KANG Ji-Chuan1,2,3 JIN Rui3,4 WEN Ting-Chi1,2 HE Jing1,3 LEI Bang-Xing1,21The Engineering and Research Center for Southwest Bio-Pharmaceutical Resources of Normal Education Ministry of China,Guizhou University,Guiyang 550025,China2Guizhou Biochem-engineering Research Center,Guizhou University,Guiyang 550025,China3Guizhou Key Laboratory of Agricultural Bioengineering,Guizhou University,Guiyang 550025,China4College of Life Science,Guizhou University,Guiyang 550025,China;Recent research advances on endophytic fungi producing taxol[J];Mycosystema;2011-02
10 Hua Yongli Ouyang Shaohua Chen Meilan Lin Shufang Huang Luai (Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700);Advances of Medicinal Endophyte Fungi Studies[J];World Science and Technology-Modernization of Traditional Chinese Medicine and Materia Medica;2008-04
China Proceedings of conference Full-text Database 3 Hits
1 CHEN Ye, FAN You-fu, PENG Qin, FANG Liang College of Life Science, Jiujiang University, Jiujiang, Jiangxi 332000, China;Preliminary Study on Diversity of Endophytic Fungi in Magnolia officinalis subsp. biloba[A];[C];2006
2 XIE Li-hua,WANG Guo-hong,YANG Min-he College of Life Science,Fujian Normal University, Fuzhou, Fujian 350007, China;Endophytic Fungi and Their Effects on the Ecological Adaptability of Host Plants[A];[C];2006
3 ZHAN Shou-fa,GANJin-lian,FAN You-fu,PENG Qin,CHEN Ye (College of Life Science,Jiujiang University,Jiujiang,Jiangxi 332000,China);Study on Endophytic Fungi in Pteris multifida Poir. Ⅰ——Isolation and Identification of Endophytic Fungi[A];[C];2007
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 MAO Zhi qun,ZHANG Wei,MA Wen (College of Food Science and Technology, Agricultural University of Hebei, Baoding 07100, China);The utilization of molecular biology in yeast taxonomy[J];Journal of Agricultural University of Hebei;2002-S1
2 TANG Xue-hui,MAO Kai,GAN You-min,LIU Lin(Department of Grassland Science,Sichuan Agricultural University,Ya'an 625014,China);Research and application of plant endophyte[J];Grassland and Turf;2005-05
3 LI Ai-hua,FAN Ming-tao*,SHI Jun-ling(College of Food Science and Engineering,Northwest A & F University,Yangling,Shaanxi 712100,China);Isolation of Endophytes from Eucommia ulmoides Oliv. and Screening for Strains with PDG production[J];Acta Botanica Boreali-Occidentalia Sinica;2007-03
4 Enzymes During the Developing Period of Muskmelon(Cucumis melon)Qiao Yongxu1,2, Liu Shuantao1, Zhao Zhizhong1*, Xing Guoming2, and He Qiwei1(1Vegetable Research Institute, Shandong Academy of Agricultural Sciences,Shandong Provincial Key Laboratory for Biology of Greenhouse Vegetables, Jinan, Shandong 250100 China; 2College of Horticulture, Shanxi Agricultural University, Taigu, Shanxi 030801 China);Study on the Correlation of Sugar Accumulation and Sucrose-metabolizing Enzymes During the Developing Period of Muskmelon(Cucumis melon)[J];Journal of Fruit Science;2004-05
5 DU Hong-Yan (Non-timber Forestry Research and Development Center of CAF, Zhengzhou 450003,China);The Progress in Research of the Active Component from Eucommia ulmoides and Its Pharmacology[J];Economic Forest Researches;2003-02
6 Qiu Deyou Huang Meijuan Fang Xiaohua Zhu Cheng (Life Science college, Peking University, Beijing 100871) Chu Chicing (Intiiuete of Botany, Academia Sinica, Beijing 100044);ISOLATION OF AN ENDOPHYTIC FUNGUS ASSOCIATED WITH TAXUS YUNNANENSIS ET L.K.Fu[J];Mycosystema;1994-04
7 LI Gui-Ling WANG Jian-Feng HUANG Yao-Jian ZHENG Zhong-Hui SU Wen-Jin (School of Life Sciences, Xiamen University, The Key Laboratory of Ministry of Education for Cell Biology and Tumor Cell Engineering, Xiamen 361005);SCREENING OF ENDOPHYTIC FUNGI WITH ANTITUMOR ACTIVITIES[J];Mycosystema;2001-03
8 SUN Jian-Qiu ZHOU Dong-Po MA Yu-Chao LI Jing XIE Yu-Hong ZHANG Peng (College of Life Science and Engineering, Qiqihar University, Qiqihar 161006) PING Wen-Xiang (College of Life Science, Heilongjiang University, Harbin 150080);INACTIVATED PARENTS PROTOPLAST FUSION OF NODULISPORIUM SYLVIFORME[J];Mycosystema;2002-03
9 XU Xue-Feng1 LIN Fan-Xue1,2 CHENG Shui-Ming1,3LIAn-Zheng1 LIN Fang-Can1 ? (1State Key Laboratory of Agricultural Microbiology, The institute ofAppliedMycology,HuazhongAgriculturalUniversity, Wuhan 430070;Department of Biology,JiningTeacherCollege,Jining 272125; National Engineering Research Center for Wheat, Henan Agricultural University Zhengzhou 450002);GENETIC DIVERSITY OF NATURAL GERMPLASM OF LENTINULA EDODES IN CHINA INFERED FROM rDNA SEQUENCES[J];;2005-01
10 Zhang Mingwei,Chen Encheng,Zhang Yan,Zhang Ruifen,Chi Jianwei,Wei Zhencheng,Tang Xiaojun(Key Laboratory of Functional Food of Ministry of Agriculture,Biotechnology Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510640, China);Effect of technological conditions on γ-aminobutyric acid accumulation in germinated indica black rice[J];Transactions of the Chinese Society of Agricultural Engineering;2007-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved