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《Journal of Northwest A & F University(Natural Science Edition)》 2011-01
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Isolation,identification and growth conditions of endophytic fungi of Eucommia ulmoides Oliv. for production of PDG

LIU Chao1,SHI Jun-ling1,ZHOU Xiao-juan1,YANG Bao-wei1,DOU Xing-xia2(1 College of Food Science and Engineering,Northwest A&F University,Yangling,Shaanxi 712100,China;2 Linyi Institute of Product Quality Supervision & Inspection,Shandong Province,Linyi,Shandong 276001,China)  
【Objective】 The study aimed at isolating pinoresinol diglucoside(PDG)-producing endophytic fungi from Eucommia ulmoides Oliv.to identify the strain with the highest yield of PDG and to investigate the basic conditions for its growth.【Method】 Endophytic fungi were isolated from the surface sterilized bark of Eucommia ulmoides Oliv.and then,cultivated in liquid broth at flask level to test the PDG production.The strain with the highest PDG production was selected and identified based on the morphological characteristics and the sequence of rDNA in the ITS district.Basic growth conditions of the strain were also studied in aspects of temperature,pH,carbon source and nitrogen source by the single-factor experimental design.【Result】 Eighteen strains of endophytic fungi were isolated from the bark of Eucommia ulmoides Oliv.Only three of them had the ability to produce of PDG.The strain XP-8,with the highest PDG production of 11.65 mg/L,was identified as Phomopsis sp.It could grow in a wide range of conditions from 20 ℃ to 35 ℃ and pH 4 to 8 with the best growth at 28 ℃ and pH 5.The strain XP-8 could use glucose and maltose,but not malic acid,among the tested carbon sources.Sodium nitrate showed to be the most favorable one among the tested nitrogen sources,whereas aspartic acid could not be used at all.【Conclusion】 The PDG-producing endophytic fungi were isolated from Eucommia ulmoides Oliv..The best strain having the highest PDG production was identified,and the basic conditions for its growth were also studied.
【Fund】: 陕西省科技攻关项目(2008K02-09);; 全国大学生科技创新项目(G07036)
【CateGory Index】: S567.19
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