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《Journal of Northwest A & F University(Natural Science Edition)》 2019-01
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Comparison of cold resistance of 8 alfalfa varieties

ZHU Aimin;ZHANG Yuxia;WANG Xianguo;TIAN Yonglei;CONG Baiming;ZHANG Qingxin;College of Agriculture,Inner Mongolia University for the Nationalities;College of Animal Science and Technology,China Agricultural University;  
【Objective】This study tested the determination method for cold resistance of 8domestic alfalfa varieties to provide theoretical basis for screening cold resistant alfalfa varieties in Horqin area.【Method】In this experiment,the annual overwintering organs of 8alfalfa varieties(Caoyuan No.2,Caoyuan No.3,Dongmu No.1,Nongjing No.1,Nongjing No.8,Zhaodong,Longmu No.801,and Gongnong No.1)were treated at low temperatures of-10,-15,-20,-25,-30and-35℃,respectively.The control was stored at 4℃.The survival rates in different treatments were measured in pot experiment,the relative conductivity and root neck vitality were measured,and the semi lethal temperature(LT50)was calculated.Logistic regression equation was also used for cluster analysis of LT50.Cluster analysis of cold re-sistance of 8alfalfa varieties by fitting Logistic regression equation.【Result】The survival rates of Dongmu No.1and Caoyuan No.3were better,but the survival rates of Caoyuan No.2and Nongjing No.8were poor.The relative electrical conductivity of root neck of 8alfalfa varieties increased with the decrease of temperature,while the vigor of root neck decreased.According to the Logistic regression equations obtained by conductance method,the LT50 of the 8alfalfa varieties showed that the cold resistance was in the order of Caoyuan No.3Dongmu No.1Nongjing No.1Gongnong No.1ZhaodongLongmu No.801Caoyuan No.2Nongjing No.8.According to the Logistic regression equation,the LT50 obtained from root neck vigor method indicated that the cold resistance was in the order of Dongmu No.1Caoyuan No.3Nongjing No.1Gongnong No.1Longmu No.801ZhaodongNongjing No.8Caoyuan No.2.The root viability method was more reliable than conductivity method,and the integrated cluster method was more representative and accurate.The 8alfalfa varieties were divided into 3categories.Dongmu No.1,Caoyuan No.3and Nongjing No.1were high cold resistant varieties,Gongnong No.1,Zhaodong and Longmu No.801 were general cold resistant varieties,while Caoyuan No.2and Nongjing No.8alfalfa were low cold resistant varieties.The survival rates of 8alfalfa varieties under different low temperature treatments were consistent with cold resistance.【Conclusion】The combined method of conductance or root neck activity with Logistic equation was feasible to calculate LT50 of alfalfa and it could be used for evaluating cold resistance of alfalfa.
【Fund】: 国家公益性行业(农业)公益项目(201403048-2);; 内蒙古自然科学基金项目(2014MS0315);; 内蒙古自治区饲用作物工程技术研究中心开放基金项目(MDK2016019)
【CateGory Index】: S541
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