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《Journal of Northwest A & F University(Natural Science Edition)》 2019-01
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Optimization of sporulation conditions for Streptommyces galilaeus AF1

ZHOU Si;HU Xiaoyun;ZHAO Pengfei;NIU Yifang;LIAO Meide;Key Laboratory of Natural Pesticide and Chemical Biology,Ministry of Education,South China Agricultural University;  
【Objective】This study explored the optimal sporulation conditions for Streptommyces galilaeus AF1.【Method】The solid fermentation conditions of spores production of S.galilaeus AF1 were studied.Single factor experiments were firstly conducted to screen the solid sporulation substrates(rice,corn flour,cassava residue,soybean flour,bran and oatmeal).Then,the effects of incubation time,temperature,liquid to solid ratio,inoculation amount,carbon source and nitrogen source on sporulation yield of S.galilaeus AF1 were tested.The conditions affecting the sporulation yield were optimized by orthogonal test based on single factor experiments.【Result】The best substrate was rice and the best fermentation conditions were incubation temperature 28℃,incubation time 6d,inoculation amount 10%(2mL spore suspension inoculated in 20 g rice),liquid(mL)to solid(g)ratio 1∶1,1.5%(m/m)glucose as carbon source,and 1.5%(m/m)peptone as nitrogen source.Under above conditions,the conidia amount reached 3.87×109 g-1,which was 29% higher than original medium.【Conclusion】The obtained S.galilaeus AF1 had high sporulation level and met the requirement of large-scale inoculum.
【Fund】: 广东省农村科技领域项目(2014A020208088)
【CateGory Index】: S476.12
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