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Studies on Goat Oocytes in vitro Maturation

Zhang Yong Liu Zelong Li Yuqiang (College of Animal Science and Veterinary Medicine,Northwestern Agricultural University,Yangling,Shaanxi 712100)  
The effects of FCS,culture time,17β estradial,FSH and LH on goat oocytes in vitro maturation were studied.The results suggested that an addition of 10%-15% FCS,1-2 mg·L -1 17β E 2,20-50 mg·L -1 LH and 10 mg·L -1 FSH was effective to improve oocyte nuclear maturation,and to extrude the first polar body.The maturation rate of oocytes cultured for 20,24 h was significantly higher than that of 16 h( P 0.05).After nuclear maturation,culture for 3-4 h was beneficial to oocyte cytoplasm maturation,and had a significant effect on fertilization,cleavage and embryo development.An addition of 10%-15% FCS,10 mg·L -1 FSH 10-50 mg·L -1 LH and 1-2 mg·L -1 17β E 2 in the TCM199 medium was an ideal culture system for goat oocyte in vitro maturation.
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