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《Chinese Journal of AIDS & STD》 2010-02
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A non-labeled real-time PCR assay targeting OmpA-VS2 for detection of Chlamydia trachomatis

LI Jian-hong, YIN Yue-ping, ZHONG Ming-ying,et al. ( Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College/National Center for STD Control, China CDC, Nanjing, Jiangsu 210042,China. )  
Objective To establish a non-labeled real-time PCR assay targeting OmpA-VS2 for detection of Chlamydia trachomatis (C. trachomatis). Methods Two pairs of universal primers were designed to amplify the OmpA-VS2 region of fifteen genotypes of C. trachomatis by the nested real-time PCR. The sensitivity and specificity of the assay were analyzed and further validated with clinical positive specimens. Results All 15 genotypes of C. trachomatis were successfully amplified with a product of 168bp. The detection limit with the assay was 1 copy per reaction mixture and no cross reactions with 10 other urogenital pathogens or commensals were found. The clinical validation reveals a coincidence with the results detected by plasmid PCR. Conclusion The assay is characterized with high sensitivity and specificity, and no separated post-PCR analysis is needed. It would be an appropriate assay to be used for detection of chlamydial infection in clinical practice as well as in disease control programs.
【Fund】: 联合国开发计划署/世界银行/世界卫生组织热带病研究与培训特别规划署(TDR)项目(项目编号A70578)~~
【CateGory Index】: R450;R697
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 YANG Bin,XUE Xiu-juan,ZHENG He-ping,PENG Hui-min,CHEN Hui,XUE Yao-hua,WU Xing-zhong(Guangdong Provincial Centers for Skin Diseases and STIs Control and Prevention,Guangzhou 510500);Molecular Epidemiology of Cryptic Plasmid of Chlamydia trachomatis in Guangzhou[J];Southern China Journal of Dermato-Venereology;2009-01
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 ZHENG He-ping JIANG Li-fang XUE Yao-hua FANG Dan-yun WU Ya-an HUANG Jin-mei Zhongshan Medical College,Sun Yat-sen University,Guangzhou 510080,China;Detection and genotyping of Chlamydia trachomatis by a reverse dot blot procedure in urogenital specimens[J];Chinese Journal of Dermatology;2006-05
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