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《Chinese Journal of AIDS & STD》 2010-05
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Construction of a HIV-1 B/C recombinant subtyped pseudovirus and evaluation of its activities

TIAN Ya-kun,WANG Wen,JIAO Yan-mei,et al.(Department of Infectious Disease,Capital Medical University Beijing Youan Hospital,Beijing 100069,China)  
Objective To extract viral DNA from the CD+4 T cells of the HIV-1 infected patients and to construct HIV-1 pseudotyped virus,which will serve as a useful tool for neutralization assay.Methods The blood samples were collected from HIV-1 infected patients and the peripheral blood mononuclear cells were separated.The provirus DNA was extracted and gp160 was amplified by PCR.The Env containing PCR amplicons was ligated into the pcDNA3.1(+) expression vector after appropriate restriction enzyme digestion.And then a pseudovirus-based neutralization assay was established.Results HIV pesudovirus containing luciferase gene was capable of infecting Tzm-bl cells.Conclusion The B/C recombinant subtyped pseudovirus has been successfully constructed and a platform to use a single-round replicative pseudovirus has been established.
【Fund】: 国家科技重大专项(2008ZX10001-006);; 北京市科委重大项目(D09060030405912)资助~~
【CateGory Index】: R512.91
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1 JIAO Yan-mei,WU Hao(Department of Infectious Diseases,Beijing Youan Hospital,Capital Medical University);Progresses in the Mechanism of HIV-1 Latency Formation[J];Journal of Capital Medical University;2009-05
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