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Quantitative analysis of oxymatrine and qualitative analysis of the other three alkaloids in the four kinds of seeds of leguminous plants

ZHOU Junhui,WANG Daqi,LI Sifeng (Institute of Botany of Shaanxi Province,Medicinal Plant Laboratory of Xi'an Botanical Garden,Xi'an 710061,China)  
Objective To compare the quantitative analysis of oxymatrine and the qualitative analysis of the other three alkaloids in the four kinds of seeds of leguminous plants and to provide some evidence for the further utilization of the above medicinal plants.Methods The separation was performed on a Lichrospher and Phecda C8 column(150 mm×4.6 mm,5 μm),using 0.05 mol·L-1 NaH2PO3-methanol-sodium perchlorate(850∶150∶10) as the mobile phase.The detection wavelength was set at 215 nm.The flow rate was 1.0 mL·min-1,and the column temperature was room temperature.Results There was a linear correlation over the range of 0.2-1.2 μg.The results showed that the average contents of oxymatrine in the seeds of Sophora alopecuroides L.,Sophora viciifolia Hance,Sophoraflavescens Ait.were 1.5%,1.5% and 2.5%,respectively;The concentration of oxymatrine in the seeds of Sophora moocroftiana was the highest,with the average 4.69%.Conclusion The seeds of Sophora viciifolia Hance and Sophora moocroftiana were ideal raw material for producing oxymatrine.
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