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Content determination,antioxidant activity estimation and docking study of ellagic acid in Rubus amabilis

DIAO Yulin;ZHU Dongqing;LIU Bin;School of Traditional Chinese Medicine,Beijing University of Chinese Medicine;National Engineering and Technology Research Center of Chirality Pharmaceutical,Shandong Lunan Pharmaceutical Group Limited Company;  
Objective To establish a method to determine the content and to estimate the antioxidant activity of ellagic acid in Rubus amabilis.Methods The RP-HPLC separation was performed on a SunFireTMC18 analytical column(150 mm×4.6 mm,5μm).The mobile phase was acetonitrile-water containing 4 mL·L-1 H3 PO4(20∶80)with a flow rate of 1.0 mL·min-1.The detection wavelength was set at 254 nm,and the column temperature was 35℃.The computer molecular simulation and 2,2′-diphenyl-1-picrylhydrazyl(DPPH·)antioxidation in vitro methods were used together to estimate the antioxidant activity of ellagic acid.Results The liner range of ellagic acid was 0.081 92-0.409 60μg(r=0.999 7,n=6).The average recovery(n=6)was 99.51%(RSD=2.00%).Ellagic acid was functioned at the-NH2 groups in receptor proteins by the hydrogen bonds between-OH groups andπ-πbonds among benzene rings,which resulted in the more significant antioxidant activity with vitamin C.Conclusion The method is simple,stable and accurate with a good reproducibility,and can be used as a method for quality control and evaluation of the main antioxidation component,ellagic acid,in Tibetan medicine Rubus amabilis.The method can be used in the quality evaluation of the further development of the plant.
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