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Purification and antioxidant activity of polysaccharide from Cicer arietinum

LI Jian;JIN Lei;HONG Zhanmei;REN Yingling;CHEN Huihui;Department of Pharmacy,the 105th Hospital of PLA;  
Objective To isolate and purify the homogeneous polysaccharides fromCicer arietinumand to evaluate the antioxidant activity.Methods After the crude polysaccharides were obtained from the fruits of Cicer arietinum by water extraction,the polysaccharides were purified by DEAE-Cellulose 52 and Sephacryl S-300 to give the homogenous polysaccharide.High performance gel permeation chromatography(HPGPC)proved that the polysaccharides were homogenous.The carbohydrate content of polysaccharide was measured by phenol-sulfuric acid method.The monosaccharide composition of the homogenous polysaccharide was analyzed by GC and GC-MS after hydrolysis.The antioxidant activity of the homogenous polysaccharide was measured by DPPH·scavenging.Results The results showed that the polysaccharides(CA-1 b)isolated from Cicer arietinum was homogeneous with relative molecular weight of 1.33×106 Da.CA-1 bwas made up of Ara,Man,Glc and Gal.And their ratio was 2.2︰3.1︰6.2︰1.0.DPPH· scavenging experiments showed that the CA-1 bhad a good antioxidant activity and there was a certain relationship between the activity and concentration with IC5018.2μg·mL-1.Conclusion These results provide a theoretical basis for further research and development of Cicer arietinum.
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