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The relationship between drug resistance mechanism of macrolides and bacterial virulence in Streptococcus pneumoniae with epidemic genotypes

ZHOU Zhimin;HE Qunli;Department of Microbiology,School of Basic Medical Sciences,Xinxiang Medical University;Xuchang People′s Hospital;  
Objective To study the relationship between the drug resistance mechanism of macrolides and bacterial virulence in invasive pneumococcal disease(IPD)with epidemic genotypes.Methods A total of 12 strains of IPD from June 2013 to June 2016 were collected as observation group,and other 10 strains of carrying Streptococcus pneumonia(S.pn)were used as control group.Then the serum genotypes were compared by multiplex PCR amplification method,and the mutation of base sequences of virulence genes including cps2 A,bacteriocin,psa A,pcs B,ply and clp P were analyzed by PCR.The drug resistance rates and minimum inhibitory concentrations(MIC)of penicillin,cefepime,levofloxacin,erythromycin and meropenem were determined by MIC method.Results The rates of 14,6 A/6 Band 19 Fgenotypes in the two groups showed no statistical difference(P0.05),while the 19 A,6 Cand17 Fin observation group were significantly higher(P0.05).The rates of six virulence genes in observation group were higher(P0.05).The insensitive rates of penicillin and erythromycin in observation group were larger(P0.05),while the sensitive rates and MIC values of the others showed no statistical difference(P0.05).Conclusion The serum genotypes of IPD were 19 A,6 Cand 17 F,and six types of virulence genes and drug resistance of penicillin and erythromycin.
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