Effect of Electroacupuncture on the Number of BSI-B4/IL-1R Double Labeled Nerve Fibers in the Dermal and Subcutaneous Tissues around the Ankle Joint in Adjuvant Arthritis Rats
CHEN Di, TANG Jun-rui, CHEN Jun, AI Bo, ZENG Qing, LI Man, WANG Li-na, LI Ling-li, LIN Chuan-you, ZHANG Jing, CAO Fu-yuan, SHI Jing, GUAN Xin-min (Tongji Medical College of Central-China University of Science and Technology, Wuhan, 430030)
Objective:To observe the effect of electroacupuncture (EA) on the number of Bandeiraea Simplicifolia I (BSI)-B4 labeled and IL-1 receptor I immunoreaction (IR) positive nerve fibers of the dermal and subcutaneous tissues around the ankle joint in adjuvant monoarthritis rats. Methods: 28 male SD rats (180～200 g) were randomized into control group, model group, IL-1 receptor antagonist (IL-1-RA) group and EA group, with 7 cases being in each group. Adjuvant monoarthritis model was induced by injection of 50 μL complete Freund's adjuvant (CFA) into the left hind ankle joint cavity. EA (4～16 Hz, 0.5～1.5 V and intermittent waves) was applied to “Huantiao”(GB 30) and “Yanglinquan”(GB 34) for 30 min following injection of CFA and on the 3rd day. In IL-1-RA group, on the 1st and 3rd day after injection of CFA, IL-1RA (1 μg/mL, 50 μL) was injected into the subcutaneous tissues around the left hind ankle joint. On the 3rd day, the rats were anesthetized with 20% urethane (1 g/kg) and transcardiac perfusion was performed by using 4% paraformaldehyde phosphate buffer (pH 7.4). The skin and subcutaneous tissues around the focus region were taken to be cut into sections (10 μm) which were then treated with BSI-B4 staining (for displaying C-fibers) and IL-1RI immuno-fluorescent labeling techniques. The number of BSI-B4/IL-1RI double labeled IR-positive nerve fibers (30 sections/rat,38.44 mm2/section) was calculated and analyzed by using fluorescent microscope and image analysis system. Results: 1) The double labeled (BSI-B4/IL-1RI/IR positive) fibers mainly distributed in the dermal and subcutaneous tissues around the ankle joint. 2) Compared with control group, BSI-B4 labeled fibers and IL-1RI/IR-positive fibers, and the double labeled fibers and the percentage of double labeled fibers/ BSI-B4 or IL-1RI single-labeled fibers in model group, IL-1-RA and EA groups were significantly higher(P0.05～0.01); 3)In comparison with model group, the number of BSI-B4 labeled fibers, IL-1RI IR-positive fibers, double stained fibers and the percentage of double-stained fibers/IL-1RI single-stained fibers of IL-1-RA group was markedly lowered(P0.05～0.01; 4)Compared with IL-1-RA group, the number of BSI-B4 labeled fibers, double labeled fibers and the percentage of double-labeled fibers / IL-1RI IR single-stained fibers of EA group was markedly lowered(P0.05), and the percentage of double labeled fibers/BSI-B4 labeled fibers was significantly higher(P0.05). Conclusion: 1) There is IL-1RI expression in the C-fibers of the dermal and subcutaneous tissues around the ankle joint in normal rats, which is upregulated in the inflammatory tissue induced by CFA. The analgesic effect of EA is probably related to its suppressive effect on C-fibers' proliferation and IL-1β(inflammation factor) induced activation of IL-1RI and its promotion effect on the synthesis of IL-1RA (the endogenous anti-inflammatory factor) in the inflammation focus.
【Fund】： 国家自然科学基金重大研究计划项目资助 (90 2 0 90 0 9) ;; 湖北省自然科学基金资助项目 (2 0 0 2AB12 4) ;; 华中科技大学医工、医理学科交叉科研基金资助项目。
【CateGory Index】： R245
【CateGory Index】： R245