Influence of Connexin 43 Gene Knockout on the Analgesic Effect of Acupuncture in Visceral Pain Mice
YU Wei-chang,HUANG Guang-ying,ZHANG Ming-min,WANG Wei1(Institute of Integrated Traditional Chinese & Western Medicine,Tongji Hospital Affiliated to Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China;1Department of Neurology,Tongji Hospital Affiliated to Tongji Medical College,Wuhan 430030)
Objective To explore the possible central and peripheral mechanisms of connexin 43 gene knockout in acupuncture analgesia.Methods Fifty-four wide type(WT) mice and 54 connexin 43 gene knockout(heterozygote,HT) mice were randomly divided into WT control group,WT model group,WT acupuncture group,HT control group,HT model group and HT acupuncture group(n=18/group).Visceral pain model was established by intraperitoneal injection of 0.6% acetic acid(0.1 mL/10 g)."Zhongwan"(CV 12) and bilateral "Zusanli"(ST 36) were punctured with filiform needles and stimulated for 30 min by manipulating the needle for 30 s every 5 min.The latency and the number of body-writhing response were observed and the contents of β-endorphin(β-EP) in hypothalamus and serum prostaglandin E2(PGE2) were detected with radioimmunoassay(RIA).Results There was no significant difference in the latency and the number of body-writhing response,and contents of hypothalamic β-EP and serum PGE2 between HT and WT control groups(P0.05).Compared with the corresponding control groups,the latency of body-writhing in WT model and HT model groups shortened significantly and the number of bodywrithing increased considerably in two model groups(P0.01).While in comparison with WT model group,the latency of bodywrithing prolonged significantly and the number of body-writhing decreased apparently in WT acupuncture group(P0.01);but no marked differences were found between HT model and HT acupuncture groups in these two indexes(P0.05).Compared with the corresponding control groups,the contents of both β-EP and PGE2 increased obviously in WT model and HT model groups(P0.05).In comparison with WT model group,β-EP levels in WT acupuncture group increased further significantly(P0.05),and serum PGE2 in WT acupuncture group decreased obviously(P0.05);but no significant changes were found between HT model and HT acupuncture groups in β-EP and PGE2 levels(P0.05).Conclusion Acupuncture has a marked antinociceptive effect in WT mice with visceral pain,which may be related to its effects in increasing hypothalamic β-EP and decreasing serum PGE2;while in connexin 43 gene knockout mice,all the above-mentioned effects of acupuncture are eliminated,indicating an important role of connexin 43 in the analgesic effect of acupuncture.
【Fund】： 国家自然科学基金重大研究计划(No.90209009);; 国家重点基础研究发展计划(“973”计划 No.2006CB504502)资助项目
【CateGory Index】： R245.311
【CateGory Index】： R245.311