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《Acupuncture Research》 2017-06
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Effects of Acupotomy Therapy on mRNA Expressions of Bcl-2,Bax,Caspase-3 in Posterior Cervical Extensor Muscles in Cervical Spondylosis Rabbits

LIU Fu-shui;ZHOU Fan-yuan;ZHANG Yi;GUO Chang-qing;School of Moxibustion,Jiangxi University of Chinese Medicine;School of Acu-moxibustion and Tuina,Beijing University of Chinese Medicine;  
Objective To observe the effect of acupotomy therapy on mRNA expressions of Bcl-2,Bax,Caspase-3 in posterior cervical extensor muscles in cervical spondylosis rabbits,and explore its mechanisms for apoptosis of cervical muscles.Methods New Zealand rabbits were randomly divided into normal,model,electroacupuncture(EA)and acupotomy groups,6 in each group.The cervical spondylosis model was established by forced head-bowing for a long term.After model establishment,acupotomy was used at the trapezius muscle starting point and attachment site of sternocleidomastoid muscle,etc.,once a week,total 3 times.EA was used at"Tianzhu"(BL 10),"Jingbailao"(EX-HN 15)and"Dazhu"(BL 11)for 3 weeks,20 min a time,3 times a week.Bcl-2,Bax,Caspase-3 mRNA expressions in posterior cervical extensor muscles were detected by real-time PCR.Results There was no significant difference in the expression of Bcl-2 mRNA among all the groups(P0.05).Compared with the normal group,Bax mRNA increased in the model group(P0.01),and that in the acupotomy group was lower than that in the model group(P0.01).The ratio of Bcl-2/Bax mRNA in the model group decreased significantly compared with that in the normal group(P0.01);in comparison with the model group,the ratio in the acupotomy group increased(P0.01);and that in the acupotomy group was higher than the ratio in the EA group(P0.05).The Caspase-3 mRNA expression in the model group increased compared with that in the normal group(P0.05),and its expression in the acupotomy group decreased compared with those in the model and EA groups(P0.05).Conclusion Acupotomy therapy can regulate the mRNA expressions of Bax and Caspase-3,and retard apoptosis in posterior cervical extensor muscles,therefore the strained muscles are relieved,which may be one of its mechanisms for improving cervical spondylosis.
【Fund】: 国家自然科学基金(No.81560792);; 江西省教育厅科技计划项目(重点项目 No.GJJ 150834);; 江西省卫生计生委中医药科研项目(重点课题 No.2016Z03)
【CateGory Index】: R245
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